HT Supplement 100X, Animal-Origin-Free

CCM-RG-0087 is a 100X HT Supplement supplied as a sterile-filtered liquid for cell culture reagent workflows. The product is animal-origin-free and formulated with [+]10 mM hypoxanthine monosodium and [+]1.6 mM thymidine. These components are commonly used to support nucleotide salvage pathway requirements in cell culture systems where defined supplementation is preferred over serum-derived or undefined inputs. The supplement is designed for controlled addition to compatible basal media during research, process development, and production-scale culture operations.

Key release specifications include a pH range of 9.2 to 10.3 and osmolality of 10 to 30 mOsmol/kg. The low osmolality profile is typical for concentrated additive solutions and should be considered in final medium formulation calculations, especially where osmolality targets are tightly controlled. Supplied in a 500 mL bottle, this reagent is suitable for laboratories and bioprocess groups that require lot-level traceability, consistent concentration, and procurement-ready documentation. For adjacent additives and formulation components, see the cell culture reagents category.

• Supplementation of mammalian cell culture media where hypoxanthine and thymidine are required as defined small-molecule additives.
• Support of hybridoma culture and antibody-producing cell culture workflows that use HT-containing media systems after selection-stage processes are complete.
• R&D evaluation of media formulations in which animal-origin-free inputs are required for improved component control and supply-chain consistency.
• Process development studies requiring a 100X liquid concentrate that can be aseptically added to prepared media at validated internal ratios.
• Scale-up preparation of supplemented media for shake flasks, spinner cultures, bench-scale bioreactors, and other controlled cell culture platforms.
• Replacement or harmonization of HT-containing supplements across multi-site laboratories using a defined 10 mM hypoxanthine monosodium and 1.6 mM thymidine concentrate.

Store CCM-RG-0087 at 2 to 8 °C protected from light. The product is shipped on blue ice to help maintain refrigerated transport conditions; upon receipt, inspect the bottle and confirm that the solution is clear and packaging is intact before inventory release. Do not freeze unless an internal validation study supports that handling approach. Use aseptic technique when removing aliquots, and minimize repeated warming, light exposure, and open-container time. The stated shelf life is 24 months when stored unopened under the recommended conditions. As with all concentrated supplements, final use concentration, compatibility, and in-process hold times should be verified within the buyer’s own media preparation procedure.

This supplement is manufactured for research and bioprocessing supply under ISO-aligned quality practices, with sterile filtration used as the terminal processing step for microbial control. CellCultureMedia can provide lot-specific documentation, including Certificate of Analysis on request, to support supplier qualification, material onboarding, and batch record requirements. Buyers may contact the team through request a quote for volume pricing, recurring supply planning, and standing-order discussions. Additional information on documentation practices is available through the quality resources page. Standard ordering is available for the 500 mL configuration at $1,625, with free worldwide shipping included where service lanes are available.