Sterile 1 M HEPES Buffer, 100 mL

HEPES, or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, is a widely used Good's buffer selected for its useful buffering range near physiological culture pH and its low tendency to participate in many common biochemical reactions. CCM-RG-0001 is supplied as a 1 M ready-to-use liquid concentrate for dilution into cell culture media, balanced salt solutions, and process buffers when additional pH control is required outside standard bicarbonate buffering systems.

This 100 mL format is designed for laboratories and process development groups that need a sterile, animal origin-free buffer with defined release specifications. The product is sterile-filtered, with pH specified at 7.2–7.5, osmolality at 1200–2020 mOsmol/kg, and endotoxin controlled to ≤1 EU/mL. As part of our cell culture reagents portfolio, it supports consistent procurement for R&D, upstream process development, analytical support, and academic core facilities.

• Supplementing mammalian cell culture media where additional buffering capacity is needed during incubator handling, bench work, imaging, sampling, or transport between instruments.
• Formulating research buffers, wash solutions, and assay support reagents that require a 1 M HEPES stock with sterile-filtered quality and animal origin-free classification.
• Supporting process development studies in which pH drift, CO2 exposure, or open handling time must be managed through a chemically defined buffer component.
• Use in suspension or adherent culture workflows after appropriate dilution, qualification, and compatibility testing by the end user.
• Standardizing reagent sourcing for laboratories that require lot traceability, controlled endotoxin, and documented pH and osmolality specifications.

Store CCM-RG-0001 at 2–8 °C and protect the container from unnecessary warming, contamination, and repeated open handling. The product is supplied as a sterile liquid and should be handled using aseptic technique after opening; do not use if the container seal is compromised, the solution shows unexpected turbidity, or the material has been stored outside validated conditions. Shelf life is 24 months when stored unopened under recommended conditions. Shipments are packed on blue ice to maintain refrigerated transit conditions, and receiving teams should transfer units to controlled 2–8 °C storage promptly upon arrival.

CellCultureMedia supplies CCM-RG-0001 for professional research and bioprocessing environments that require consistent specifications and procurement-ready documentation. Manufacturing and release controls are ISO-aligned, with sterility, endotoxin, pH, osmolality, appearance, labeling, and shelf-life attributes managed through quality procedures. A lot-specific Certificate of Analysis can be requested through our quality documentation process. For standing orders, multi-pack requirements, or annual demand planning, submit details through request a quote. Free worldwide shipping is available where service coverage and import conditions permit.