Calcium- and Magnesium-Free DPBS, No Phenol Red

CCM-RG-0003 is a Dulbecco’s phosphate-buffered saline formulation supplied without calcium ions, magnesium ions, or phenol red. It is categorized as a balanced salt solution within cell culture reagents and is intended for routine research, development, and bioprocess support environments that require a defined, ready-to-use liquid buffer. The absence of Ca2+ and Mg2+ is useful when a protocol requires reduced cell-to-cell adhesion support before dissociation or when divalent cations may affect downstream reagent performance. The absence of phenol red also reduces background color and avoids pH indicator interference in assays or imaging-adjacent preparation steps.

The product is manufactured as an animal-origin-free liquid reagent and filter sterilized for cell culture use. Key release specifications include pH 7.0-7.4, osmolality 270-300 mOsmol/kg, endotoxin not more than 1 EU/mL, and a stated shelf life of 48 months when stored as directed. The 500 mL bottle format supports laboratory-scale consumption, media preparation support, and standardized wash steps in R&D or process development labs. For procurement planning, the listed unit price is $47.22, with quotation support available through request quote for volume requirements, standing orders, or consolidated sourcing.

• Rinsing adherent cell monolayers before dissociation, passaging, or reagent exchange where calcium- and magnesium-free conditions are preferred.
• Washing suspension cells by centrifugation and resuspension in a physiologically balanced phosphate-buffered salt solution.
• Diluting compatible cell culture reagents, concentrated supplements, or process intermediates where a clear, phenol-red-free buffer is required.
• Short-duration handling of mammalian cells during counting, sampling, transfer, or analytical preparation steps outside complete growth medium.
• Preparation of control wells, blank solutions, and wash steps in workflows where phenol red absorbance or color may complicate visual or instrumental readout.
• Supporting upstream bioprocess development, academic cell culture laboratories, and quality-controlled reagent standardization across multiple users or sites.

Store CCM-RG-0003 at 2-30 °C, protected from light. The product ships at ambient temperature, consistent with the specified storage range, and should be inspected on receipt for container integrity, clarity, labeling, and lot information before release into laboratory inventory. Keep the bottle closed when not in use and apply aseptic technique during dispensing to maintain sterility after opening. Do not use the reagent if visible contamination, unexpected turbidity, leakage, or a compromised closure is observed. Rotate inventory by expiration date and record lot numbers in batch records, laboratory notebooks, or electronic inventory systems as appropriate for traceability.

CellCultureMedia supplies CCM-RG-0003 for research and bioprocessing customers that require specification-driven sourcing, lot traceability, and responsive documentation. Production is performed under ISO-aligned quality practices, with release testing aligned to the stated sterility, endotoxin, pH, osmolality, appearance, and shelf-life specifications. A Certificate of Analysis is available on request, and additional procurement documentation can be coordinated through the CellCultureMedia team. Quality program information is available at quality. Free worldwide shipping helps simplify international purchasing for standard orders, while bulk and recurring requirements can be quoted to support forecasted demand and controlled inventory planning.