DPBS Powder without Calcium, Magnesium, or Phenol Red

CCM-RG-0004 is a powdered DPBS reagent designed for preparation of calcium-free, magnesium-free, phenol red-free balanced salt solution. The formulation is appropriate where divalent cations or color indicator components are not desired in wash, dilution, or handling steps. The powder format reduces storage volume compared with liquid buffers and supports centralized preparation of working solution using qualified water and controlled mixing procedures.

This 10 L pack is classified as animal origin-free and is supplied as a sterile-filtered reagent. Key release specifications include pH 7.2–7.7, osmolality 270–300 mOsmol/kg, endotoxin ≤1 EU/mL, and a stated shelf life of 48 months when stored as recommended. For related buffers and support reagents, see our cell culture reagents category. The listed 10 L pack price is $120.83, with quotation support available for scheduled or multi-site procurement.

• Routine washing of adherent or suspension cell cultures where a phosphate buffered saline system is required without calcium or magnesium ions.
• Preparation steps before enzymatic or non-enzymatic cell detachment workflows, where removal of divalent cations may improve process consistency.
• Dilution, rinsing, and temporary handling of research cell cultures during media exchange, sampling, or assay setup.
• Phenol red-free workflows where visual color indicator interference is not desirable, including absorbance, fluorescence, or imaging-adjacent protocols.
• Buffer preparation for R&D, process development, and academic laboratory operations requiring animal origin-free balanced salt solution with documented endotoxin and osmolality limits.

Store the unopened powder at 2–30 °C protected from light. The product ships at room temperature, supporting standard ambient logistics and simplified international receiving. After reconstitution, laboratories should use validated in-house procedures for water quality, mixing, container compatibility, filtration or aseptic handling as applicable, labeling, and hold-time assignment. Because the formulation contains no phenol red, visual pH color indication should not be expected; confirm working solution parameters using calibrated instrumentation when required by your protocol or quality system.

CellCultureMedia supplies CCM-RG-0004 for research and bioprocessing support use under ISO-aligned quality practices, with lot-specific documentation available on request. A Certificate of Analysis can be requested to confirm release specifications such as pH, osmolality, endotoxin, sterility-related release status, shelf life, and storage conditions. Procurement teams can submit requirements through request a quote for volume pricing, annual supply planning, and shipping coordination. Additional information about documentation practices is available on our quality page. Free worldwide shipping is available for eligible orders, subject to destination and order review.