HEPES Powder Cell Culture Buffer
HEPES powder, SKU CCM-RG-0002, is offered to distributors, resellers and bulk buyers in a 1 kg pack as a powder. Listed shelf life is 24 months, storage 2~30 ℃, protected from light. Supply is quote-only.
- ✓ Wholesale and bulk pricing on request
- ✓ Private-label and OEM packaging quoted on request
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
Wholesale supply terms
- Pack size
- Listed pack: 1 kg. Other pack sizes on request.
- Minimum order
- Minimum order quantities on request
- Bulk format
- Supplied as powder; larger bulk packs quoted on request
- Private label / OEM
- Private-label and OEM packaging quoted on request
- Lot reservation
- Lot reservation for repeat supply on request
- Lead time
- Quoted per order
- Documents
- Certificate of Analysis on request
We supply companies that resell to laboratories: distributors, resellers, catalogue companies, marketplaces and private-label buyers. We do not sell single units to individual labs.
Product summary
HEPES buffer supplied as a powder in a 1 kg pack, for preparing buffered solutions and media. It is classified as animal origin-free and ships at room temperature.
A 1 kg pack suits repackers and resellers who sell buffer in smaller units under their own catalogue.
Supply facts for resellers
- SKU: CCM-RG-0002
- Pack size: 1 kg
- Form: Powder
- Classification: Animal Origin-free
- Shelf life: 24 months
- Storage: 2~30 ℃, protected from light
- Shipping: Room Temperature
Certificate of Analysis on request.
Private label and lot reservation
Private label and lot reservation can be requested; both are quoted per project or order. See the distributor programme.
What to send for a quotation
- Product: CCM-RG-0002, HEPES powder
- Pack size: 1 kg, as listed
- Quantity per quarter, or the size of a first order
- Destination country and delivery point
Send it through request a quote.
Specifications
Storage and handling
Storage: 2~30 ℃, protected from light. Shelf life: 24 months. Shipped cold-chain where required.
Quality and documentation
Manufactured under ISO-aligned quality management systems. A Certificate of Analysis is available on request: email info@cellculturemedia.bio with the SKU.
About this product
Can we sell this product under our own label?
How is it shipped and stored?
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Learn more about this
- HEPES buffer in cell cultureHEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic buffer used in cell culture to hold medium at physiological pH without depending on incubator CO2. Its pKa is about 7.5 at 20-25 degrees C and about 7.3 at 37 degrees C, giving useful buffering across roughly pH 6.8-8.2, and it is normally added to medium at 10-25 mM from a sterile 1 M stock. Because HEPES buffering does not rely on the carbonic acid/bicarbonate equilibrium, HEPES-supplemented medium resists the fast alkaline drift that occurs when a flask leaves a 5% CO2 incubator.
- Cell culture buffersCell culture buffers hold medium in the pH 7.2-7.4 range that mammalian cells require, against the acid load cells generate as they metabolise. The default system in almost every classical medium is sodium bicarbonate working with the CO2 in the incubator atmosphere, which is why bicarbonate content must be matched to the incubator setting: about 1.5-2.2 g/L NaHCO3 for 5% CO2 and 3.7 g/L for 10% CO2. Organic buffers such as HEPES (pKa 7.48) are added at 10-25 mM to hold pH when cultures are outside a CO2 atmosphere, while phosphate-buffered solutions such as PBS and DPBS are used for washing and short-term handling rather than for growth.
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