0.5 mM EDTA Solution, Calcium/Magnesium-Free

CCM-RG-0010 is a 0.5 mM EDTA liquid reagent formulated without calcium ions, magnesium ions, or phenol red. EDTA supports cell detachment by chelating divalent cations that contribute to cell-to-surface and cell-to-cell adhesion. The absence of calcium and magnesium makes the solution suitable for workflows where cation-dependent adhesion needs to be reduced before passaging, harvesting, counting, or downstream culture processing. The phenol red-free format is useful where color indicator carryover is not desired in imaging, analytical readouts, or formulation-sensitive steps.

This 500 mL reagent is classified as animal origin-free and sterile-filtered, with an endotoxin specification of ≤1 EU/mL and a stated shelf life of 48 months when stored as directed. It is positioned within cell culture reagents for procurement programs that require defined dissociation reagents with clear documentation, lot traceability, and predictable supply. The product is supplied as a ready-to-use liquid, reducing in-house preparation time and helping teams standardize dissociation conditions across R&D, process development, and routine production support environments.

• Non-enzymatic dissociation support for adherent mammalian cell culture when a mild chelation step is preferred over protease-based release.
• Pre-passaging rinse or incubation step for monolayer cultures that require calcium- and magnesium-free conditions before detachment or harvest.
• Phenol red-free handling workflows where optical assays, imaging, or media formulation studies benefit from reduced color indicator carryover.
• Cell culture process development where animal origin-free ancillary reagents are specified for material control, supplier qualification, or scale-up planning.
• Preparation of cells for counting, reseeding, experimental setup, or transfer into defined media systems after appropriate validation by the user.

Store CCM-RG-0010 at 2 to 30 ℃ and protect the bottle from light. Maintain the container closed when not in use and apply aseptic technique during dispensing to preserve the sterile-filtered status of the liquid. Do not use the reagent if there is visible contamination, unexpected turbidity, container damage, or compromised closure integrity. For consistent results, bring the required aliquot to the operating temperature defined by the laboratory protocol, minimize repeated open-container exposure, and record lot number, opening date, and user-defined in-use hold time in the batch or laboratory record.

CellCultureMedia supplies CCM-RG-0010 for research, cell culture, and bioprocessing use with specification controls suitable for B2B procurement review. Manufacturing and release practices are ISO-aligned, and lot-specific documentation can be requested through the quality documentation channel. Key release attributes include sterile-filtered liquid form, animal origin-free classification, endotoxin ≤1 EU/mL, and 48-month shelf life under the specified storage conditions. Standard 500 mL units are available at the listed price, with free worldwide shipping and support for volume forecasts, scheduled purchasing, and bulk pricing through quotation requests.