0.25% Trypsin-EDTA Solution

CCM-RG-0043 is a 0.25% Trypsin-EDTA Solution designed for controlled dissociation of adherent mammalian cell monolayers from culture vessels, microcarriers, and related growth surfaces. The formulation combines 2.5 g/L trypsin, a proteolytic enzyme used to cleave adhesion-associated proteins, with 0.38 g/L EDTA·4Na, a chelating agent that helps reduce calcium- and magnesium-dependent cell attachment. This dual-action composition supports consistent detachment when users need a standard animal-origin digestion reagent for cell culture operations.

The product is supplied as a sterile-filtered liquid in a 500 mL format for laboratory and process-development use. Key release specifications include pH 7.2–8.0 and osmolality 270–320 mOsmol/kg, supporting compatibility with common mammalian cell culture workflows when used according to an established protocol. The reagent is categorized under cell digestion and dissociation reagents within our cell culture reagents portfolio, making it suitable for procurement teams standardizing media-adjacent consumables across R&D, pilot, and production-support laboratories.

• Routine passaging of adherent cell cultures where enzymatic detachment is required before reseeding, counting, cryopreservation, or assay setup.
• Process-development studies evaluating harvest timing, exposure duration, neutralization strategy, and post-dissociation cell recovery across defined vessel formats.
• Scale-up workflows involving flasks, multilayer culture vessels, roller bottles, or microcarrier-based culture systems, subject to user qualification for the target cell type.
• R&D protocols requiring EDTA-assisted dissociation to improve detachment efficiency compared with enzyme-only approaches in calcium- and magnesium-sensitive attachment systems.
• Preparation of single-cell suspensions for downstream in vitro assays, transfection workflows, cell banking activities, or analytical sampling where a standardized detachment reagent is specified.

Because trypsin sensitivity varies by cell line, culture age, confluency, matrix coating, and serum-free or serum-containing conditions, users should validate exposure time and neutralization method before routine implementation. For high-value or shear-sensitive cultures, a small-scale qualification run is recommended to define detachment performance, morphology, viability, and reseeding behavior under site-specific conditions. Procurement managers can reference CCM-RG-0043 when requesting repeat lots, while technical teams can align incoming inspection with pH, osmolality, sterility, storage, and shelf-life requirements.

Store CCM-RG-0043 frozen at -30 to -5 °C and protected from light. The stated shelf life is 24 months when the product remains unopened and stored under recommended conditions. The product ships on dry ice to maintain the frozen state in transit; upon receipt, inspect the container, verify labeling, and transfer promptly to qualified frozen storage. Avoid repeated freeze-thaw cycles because enzymatic performance can decline after unnecessary thermal stress. For routine use, thaw only the required volume under controlled conditions, mix gently after thawing, and use aseptic technique to reduce contamination risk. Do not warm for extended periods, and return unused aliquots to appropriate storage only if your internal procedure permits refreezing. As with other enzymatic dissociation reagents, users should confirm that temperature, contact time, and quenching conditions are compatible with the specific culture system. For multi-site supply planning or larger lot requirements, submit details through request a quote so our team can coordinate availability, shipment timing, and documentation needs.

CCM-RG-0043 is produced as a sterile-filtered, animal-origin liquid reagent for cell culture-related applications. Release information includes pH range, osmolality range, form, sterility method, storage condition, shipping condition, and shelf life, supporting technical review by quality, procurement, and process-development teams. A Certificate of Analysis is available on request for lot-specific documentation, and customers may use our quality information to support supplier qualification, incoming material review, and internal change-control files. Manufacturing and documentation practices are aligned with ISO-style quality expectations for research and bioprocessing supply chains, with attention to traceability, specification control, and consistent packaging.

CellCultureMedia supports international B2B ordering for academic, biotech, and biopharma laboratories. This 500 mL unit is priced at $201.39, and free worldwide shipping is available where order conditions and destination logistics permit. Dry-ice shipment is used for temperature control, and lead time may depend on inventory status, export routing, and cold-chain service availability. For recurring demand, users can request bulk pricing, scheduled supply, or reserved inventory to reduce sourcing variability across passaging, cell banking, and development campaigns.