Trypsin-EDTA 0.25% Without Phenol Red

CCM-RG-0044 is a 0.25% Trypsin-EDTA solution supplied without phenol red for laboratories that require a clear, indicator-free dissociation reagent. The product is a liquid, sterile-filtered formulation classified as animal origin and intended for use with adherent mammalian cell cultures where enzymatic detachment is appropriate. The absence of phenol red can simplify downstream visual assessment, assay setup, and workflows in which colorimetric interference needs to be minimized. It is part of CellCultureMedia’s cell culture reagents portfolio for R&D and bioprocess support.

Key release specifications include pH 7.2–8.0 and osmolality 270–320 mOsmol/kg, aligning the solution with common mammalian cell-culture operating ranges. EDTA supports detachment by binding calcium and magnesium ions that contribute to cell adhesion, while trypsin cleaves adhesion-associated proteins at the culture surface. The 100 mL bottle size is suitable for multiuser laboratories, method development, cell banking preparation, and scale-down process work. Because the reagent contains animal-origin trypsin, buyers should confirm suitability against internal material policies, documentation requirements, and any animal-origin-free procurement programs before implementation.

• Routine passaging of adherent mammalian cell lines grown in flasks, plates, roller bottles, or other standard cultureware.
• Preparation of single-cell suspensions for counting, seeding, expansion, transfection setup, cryostorage preparation, or analytical sampling.
• Phenol red-free workflows where optical, fluorescence, or colorimetric readouts benefit from minimizing background color contribution.
• Process development studies comparing dissociation timing, cell recovery, and post-detachment viability across culture surfaces or media systems.
• R&D and academic laboratories requiring a ready-to-use sterile liquid reagent with defined pH and osmolality ranges.
• Procurement programs that need a compact 100 mL pack size and documented supply support; for volume planning, use the request a quote workflow.

Store CCM-RG-0044 frozen at -30 to -5 °C and protected from light. The stated shelf life is 24 months when the product is stored under recommended conditions and handled using aseptic technique. Shipment is performed on dry ice to maintain frozen condition during transit; inspect the package on receipt and transfer promptly to qualified frozen storage. Thaw only the amount needed for near-term use, mix gently after thawing, and avoid repeated freeze-thaw cycles where possible. Do not use if the container integrity is compromised, if particulate contamination is observed, or if internal acceptance checks fall outside laboratory requirements.

This reagent is sterile-filtered and manufactured under ISO-aligned quality practices with controlled documentation for lot traceability. Certificate of Analysis availability can be confirmed during ordering and is recommended for regulated research, cell banking support, and process-development records. CellCultureMedia can provide specification review, quotation documentation, and logistics coordination for international purchasing teams. Free worldwide shipping is available, with dry-ice handling applied to this frozen format. For supplier qualification details, documentation expectations, or quality-system questions, review our quality information or contact the procurement team through the quotation channel.