Serum-Free Hybridoma Medium III

This serum-free hybridoma cell culture medium is formulated as a liquid, animal-origin classified medium for suspension or adapted hybridoma workflows. It contains phenol red for visual pH indication, D-glucose as a carbon source, 1.5 mM L-glutamine, and 1.25 mM L-alanyl-glutamine to support nitrogen metabolism and improved glutamine availability during routine culture. The medium is supplied ready to use and is intended for cell culture research, assay development, and bioprocessing studies where serum removal is desired for lot-to-lot consistency and simpler downstream analysis.

Key release specifications include pH 7.0-7.4, osmolality 270-340 mOsmol/kg, endotoxin not more than 3 EU/mL, and filter-sterilized manufacture. The product is designed for use at 37 °C in a humidified incubator with 5-10% CO2. For procurement teams standardizing serum-free inputs, CCM-SF-0054 provides a defined 500 mL bottle configuration with clear handling requirements and documentation support. Related defined formulations can be reviewed in our serum-free media category.

• Serum-free expansion and maintenance of hybridoma cells after appropriate adaptation from serum-containing conditions.
• Monoclonal antibody research workflows requiring reduced serum-derived background and improved analytical consistency.
• Small-scale process development, feed strategy evaluation, and culture-condition screening for hybridoma platforms.
• R&D comparison studies where pH range, osmolality, endotoxin limit, and sterility status must be controlled across lots.
• Biopharmaceutical process-support studies involving serum-free media selection, cell-growth assessment, and supernatant characterization.

Because hybridoma performance can vary by clone, users should qualify the medium with their own cell bank, inoculation density, passage schedule, and productivity criteria. Process engineers may also compare viable cell density, metabolite accumulation, and product concentration against the current reference medium before implementing the formulation in routine workflows.

Store unopened bottles at 2-8 °C protected from light. Do not freeze unless a site-specific stability protocol has been qualified, as freeze-thaw exposure may alter performance. The stated shelf life is 12 months when stored under recommended conditions and handled aseptically. Inspect the bottle before use for leakage, turbidity, precipitate, or unexpected color shift. Warm only the volume required for use, mix gently, and avoid extended exposure to room temperature or strong light. After opening, use aseptic technique and follow internal hold-time procedures appropriate for serum-free liquid media.

CCM-SF-0054 is produced under ISO-aligned quality practices with filter sterilization and lot-based release controls. Specification data include sterility status, pH, osmolality, endotoxin limit, shelf life, storage condition, and shipping condition. A Certificate of Analysis can be requested through our quality documentation portal, and procurement teams can confirm current lot availability before ordering. Standard shipment is on blue ice, and free worldwide shipping is available where service coverage permits. For volume demand, standing orders, or multi-site supply planning, submit details through request a quote.