Chemically Defined CHO Serum-Free Medium

CCM-SF-0003 is an animal-origin-free, chemically defined CHO medium in liquid form for serum-free culture. It is suitable for procurement programs that require lot-to-lot specification visibility, simplified incoming inspection, and defined raw material inputs for upstream cell culture. The formulation includes phenol red as a visual pH indicator and D-glucose as a carbohydrate source. It does not include L-glutamine or hypoxanthine-thymidine supplement, allowing process engineers to add these components separately according to feed strategy, clone requirement, or design-of-experiments protocols.

This product is part of the CellCultureMedia serum-free media portfolio and is intended for CHO-based research, scale-up studies, and bioprocess development. Source specifications include pH 7.0–7.4, osmolality 270–340 mOsmol/kg, endotoxin not more than 3 EU/mL, and filter sterilization. Recommended culture conditions are 37 °C with 5–10% CO2, subject to cell-line adaptation and site-specific process parameters.

• Serum-free culture of CHO-K1, DG44, and suspension-adapted CHO production platforms used in recombinant protein expression studies.
• Clone screening, seed train development, and upstream process optimization where chemically defined inputs are preferred over serum-containing systems.
• Fed-batch or batch development programs requiring independent control of L-glutamine, glutamine alternatives, and hypoxanthine-thymidine supplementation.
• Biopharmaceutical and vaccine antigen process research requiring animal-origin-free liquid medium and documented sterility, endotoxin, pH, and osmolality attributes.
• R&D and pilot-scale workflows that need a 500 mL pack size for screening, method transfer, qualification runs, or small-volume production experiments.

Store CCM-SF-0003 at 2–8 °C protected from light. Do not use if the container integrity is compromised, if visible contamination is observed, or if the product is outside its assigned shelf life. The stated shelf life is 12 months when stored as recommended. Shipments are dispatched with blue ice or equivalent cold-pack materials to help maintain refrigerated transport conditions. Before use, mix gently, avoid foaming, and warm only the required volume using site-approved aseptic handling procedures. If additional L-glutamine or hypoxanthine-thymidine supplement is required, add sterile components immediately before use or according to the validated local process.

CellCultureMedia supplies CCM-SF-0003 under ISO-aligned manufacturing and quality practices appropriate for research and bioprocess supply chains. Lot documentation can support receiving inspection, change control review, and internal material qualification. A Certificate of Analysis is available on request and may include sterility, endotoxin, pH, osmolality, appearance, and lot identification data where applicable. Review quality resources at CellCultureMedia quality or submit volume requirements through request quote. Free worldwide shipping is available where serviceable; temperature-managed logistics and lead times are confirmed during quotation.