MEM Non-Essential Amino Acids Solution 100X
MEM Non-Essential Amino Acids Solution 100X is an animal-origin-free, filter-sterilized liquid supplement for cell culture media preparation. Supplied as a 100 mL concentrated reagent under SKU CCM-RG-0036, it is used to enrich MEM and other compatible formulations with amino acids commonly considered non-essential for many mammalian cells. The product is stored at 2โ8 ยฐC protected from light and ships on blue ice, with free worldwide shipping available for eligible orders.
- โ Free worldwide shipping
- โ ISO-aligned manufacturing
- โ Certificate of Analysis on request
- โ Bulk & custom volumes quoted on request
Overview
This 100X MEM non-essential amino acids solution is a ready-to-use liquid cell culture additive for laboratories and bioprocess groups standardizing medium supplementation. The formulation is classified as animal-origin-free and is intended for research, development, and manufacturing support workflows where a defined amino acid supplement is preferred over in-house weighing and dissolution. It is supplied in a 100 mL pack size for routine bench-scale use, media optimization studies, and small-batch preparation.
Key supplied specifications include liquid form, filter sterilization, pH 0.6โ1.7, 24-month shelf life, storage at 2โ8 ยฐC, protection from light, and blue-ice shipment. The low pH reflects the concentrated stock solution and should be evaluated in the context of the final dilution and the buffering capacity of the complete medium. For related supplements and compatible preparation materials, see our cell culture reagents category or request a quote for project-specific supply planning.
Applications
- Supplementation of MEM-based culture media at 1X final concentration after appropriate dilution from the 100X stock.
- Support of mammalian cell culture workflows where non-essential amino acid availability is used to reduce biosynthetic demand and improve formulation consistency.
- Media development, comparability studies, and process transfer work requiring a standardized liquid additive rather than powdered component handling.
- Routine preparation of complete media for adherent or suspension cell lines when the base formulation and cell-line requirements call for MEM non-essential amino acids.
- Academic, biotech, and biopharma R&D laboratories requiring animal-origin-free cell culture additives with documented sterility processing.
Storage and Handling
Store CCM-RG-0036 at 2โ8 ยฐC and protect the bottle from light before and after opening. Do not use the reagent if the container is compromised, the solution shows unexpected turbidity, or the expiry date has passed. The product is shipped on blue ice to support refrigerated transit conditions. Use aseptic technique when dispensing, minimize repeated warming, and return the bottle to refrigerated storage promptly after use. Because this is a 100X acidic stock, add it to media using validated dilution calculations and confirm final medium pH after all components are combined.
Quality and Documentation
CellCultureMedia supplies this reagent for controlled cell culture workflows with ISO-aligned documentation practices and lot traceability. The product is filter sterilized, and a Certificate of Analysis is available on request through our quality documentation process. The source specifications provided for this item include pH, sterility method, shelf life, storage, and shipping conditions; osmolality and endotoxin limits are not stated in the supplied product data, so buyers requiring those attributes should confirm lot documentation before purchase. Free worldwide shipping is available for eligible orders, and procurement teams may contact us for bulk pricing, standing orders, or consolidated shipment planning.
Specifications
Storage & Handling
Storage: 2~8 โ, protected from light. Shelf life: 24 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation โ Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data โ is available on request. Email [email protected] with the SKU.
About this product
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- MEM Alpha (ฮฑ-MEM) and Minimum Essential MediumMEM alpha (ฮฑ-MEM) is Eagle's Minimum Essential Medium enriched with all the non-essential amino acids, sodium pyruvate, lipoic acid, ascorbic acid, biotin and vitamin B12, and is supplied either with or without ribonucleosides and deoxyribonucleosides. It uses the same salt base as MEM โ 2,200 mg/L sodium bicarbonate matched to a 5% CO2 atmosphere, 1,000 mg/L glucose and 1.8 mM calcium โ and is the standard medium for mesenchymal stromal cells, bone marrow cultures, osteoblasts, CHO-DXB11 and CHO-DG44 selection, and many primary cell types. The nucleoside-free version is required for HAT and methotrexate-based selection systems.
- Essential and Non-Essential Amino Acids in Cell CultureIn cell culture, an amino acid is called essential if cultured mammalian cells cannot make enough of it and it must be supplied in the medium - thirteen of them, the set Harry Eagle defined, including arginine, cystine, tyrosine and glutamine that are not classed as dietary essentials. The seven non-essential amino acids - glycine, alanine, asparagine, aspartic acid, glutamic acid, proline and serine - can be synthesised by most cells, and are supplied anyway because making them consumes carbon, ATP and reducing equivalents that would otherwise go into growth. A MEM NEAA 100X supplement contains all seven at 10 mM, giving 0.1 mM of each at working strength; add it to MEM or DMEM, which contain few or none, and leave it out of Ham's F-12, IMDM and DMEM/F-12, which already carry all seven.
- EMEM vs DMEM: What the Modification Actually ChangedEMEM (Eagle's Minimum Essential Medium, also sold as MEM) and DMEM (Dulbecco's Modified Eagle Medium) are the same medium one generation apart: DMEM is Eagle's formulation enriched, with roughly four times the vitamins, about twice most amino acids, twice the glutamine, added glycine and serine, ferric nitrate, and 3.7 g/L sodium bicarbonate against EMEM's 1.5-2.2 g/L. The practical consequence is that DMEM supports fast-growing, metabolically demanding lines such as HEK293 and NIH/3T3, while EMEM suits slower, less demanding adherent cells and primary lines - and because DMEM's higher bicarbonate is formulated for 10% CO2 while EMEM's suits 5%, the two are not interchangeable without checking your incubator.
