Low-Endotoxin Bovine Serum Albumin Powder
Low-endotoxin bovine serum albumin powder is an animal-origin cell culture supplement used as a defined protein component, carrier, stabilizer, and binding agent in research and bioprocess workflows. CCM-RG-0080 is supplied as a 10 g powder format for flexible formulation and controlled addition to media, buffers, and reagent systems. The product is priced at $319.44 and ships at room temperature, with free worldwide shipping available for qualified orders.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-RG-0080 is a low-endotoxin bovine serum albumin powder intended for cell culture reagent preparation and media supplementation. Bovine serum albumin is widely used where a soluble protein source is required to support adsorption control, fatty acid and small-molecule binding, reagent stabilization, and reduced non-specific interaction with plasticware or assay surfaces. The powder format gives process engineers and R&D teams control over final concentration, hydration protocol, filtration strategy, and compatibility with existing formulations.
This item is classified as animal origin and falls under cell culture supplements within the cell culture reagents category. It is suitable for organizations that require a procurement-ready BSA input with a long assigned shelf life and low-endotoxin positioning, while retaining flexibility for internal qualification. Because no fixed pH or osmolality is specified for the dry powder, those parameters should be verified in the final prepared solution or medium according to the user’s formulation and release criteria.
Applications
- Protein supplementation for mammalian cell culture media, serum-reduced formulations, and custom reagent systems where BSA is part of the approved formulation design.
- Carrier protein for dilution of growth factors, cytokines, peptides, lipids, and other labile components used in research-scale cell culture workflows.
- Blocking and surface-conditioning component for cell-based assays, plate preparation, membrane handling, and reagent-contact surfaces where reduced non-specific adsorption is required.
- Stabilizer for prepared buffers, enzyme-compatible reagent mixes, and media additives that benefit from albumin-based protection during preparation or short-term use.
- Bioprocess development studies requiring an animal-origin albumin input that can be qualified under internal raw-material assessment, supplier review, and lot documentation procedures.
Storage and Handling
Store the unopened powder at 2–8 °C protected from light. The assigned shelf life is 48 months when stored under the recommended conditions and handled using appropriate laboratory practices. The product ships at room temperature, which is suitable for transit of the dry powder; upon receipt, inspect the container, record the lot information, and transfer it to refrigerated storage. Prepare solutions with validated water or buffer systems, allow complete dissolution without excessive foaming, and use sterile filtration or aseptic processing as required by the end application. Reconstituted solutions should be stored and assigned hold times according to the user’s internal qualification data.
Quality and Documentation
CellCultureMedia supplies CCM-RG-0080 for research, cell culture, and bioprocessing use by procurement teams that need clear specifications, traceable ordering, and responsive support. Manufacturing and supply controls are managed using ISO-aligned quality practices, and lot-specific documentation such as a Certificate of Analysis can be requested through quality documentation support. Endotoxin status, appearance, and other release attributes should be reviewed against the applicable lot record before use in controlled workflows. For standing orders, larger quantities, or multi-site procurement, submit requirements through request a quote. Free worldwide shipping is available for qualified orders, helping laboratories simplify total landed cost evaluation.
Specifications
Storage & Handling
Storage: 2~8 ℃, protected from light. Shelf life: 48 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
About this product
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Learn more about this
- Endotoxin Testing in Cell CultureEndotoxin testing measures bacterial lipopolysaccharide (LPS) in laboratory water, media, sera and reagents, reported in endotoxin units per millilitre (EU/mL), where 1 EU corresponds to roughly 0.1–0.2 ng of reference-standard E. coli endotoxin. The compendial methods are the LAL (limulus amebocyte lysate) assay in its gel-clot, turbidimetric and chromogenic forms under USP <85>, and the animal-free recombinant Factor C (rFC) assay under USP <86>; kinetic formats quantify down to about 0.001–0.005 EU/mL. Practical cell-culture limits are 0.25 EU/mL for water, ≤1 EU/mL for most cell-culture-grade reagents, and ≤10 EU/mL as the general industry standard for fetal bovine serum, with low-endotoxin grades at ≤1 EU/mL and ultra-low grades below 0.1 EU/mL. Endotoxin survives autoclaving and passes 0.2 µm filters, so it must be excluded at source rather than removed later.
- Bovine Serum in Cell CultureBovine serum is the liquid fraction of clotted cattle blood, added to culture medium at 5-10% to supply growth factors, hormones, transport and attachment proteins, lipids and trace elements that basal media do not contain. Fetal bovine serum (FBS) and fetal calf serum (FCS) are two names for the same product, collected from the fetus at slaughter of pregnant cows; newborn calf serum comes from calves under about 20 days old and donor bovine serum from controlled donor herds aged roughly 12-36 months, both containing more immunoglobulin and fewer growth factors than FBS. Because serum is an undefined biological material with substantial lot-to-lot variation, unresolved animal welfare questions and a volatile supply chain, defined serum-free and animal-origin-free media are increasingly preferred where the cell line will tolerate them.
- Heat Inactivation of Fetal Bovine Serum (FBS)Heat inactivation of fetal bovine serum is a controlled 30-minute incubation at 56 degrees C that destroys the heat-labile proteins of the complement cascade before the serum is added to culture medium. The serum is thawed, brought to 37 degrees C, transferred to a 56 degrees C water bath, held for exactly 30 minutes from the moment the serum itself reaches temperature with gentle swirling every 5-10 minutes, then cooled immediately and aliquoted. For routine culture of established cell lines it is usually unnecessary, because complement activity in fetal serum is low and heating also degrades labile growth factors; it remains standard practice for immunological assays and complement-sensitive cells.
- Recombinant Human Albumin in Cell CultureRecombinant human serum albumin (rHSA) is a 585-amino-acid, 66.5 kDa non-glycosylated protein produced in yeast such as *Pichia pastoris* or in transgenic rice rather than purified from human plasma, and it is used as an animal-origin-free supplement in serum-free and chemically defined culture media. In culture it works as a carrier for fatty acids, lipids, hormones and trace elements, as an antioxidant through its free cysteine-34 thiol, as a scavenger that sequesters toxic metals and excess free fatty acids, and as a shear-protective surface-active protein in stirred and sparged culture. Typical working concentrations are 0.1-10 g/L, most often 0.5-5 g/L, and it is commonly supplied as a 200 mg/mL (20%) solution. Its performance depends heavily on what is bound to it, so fatty-acid-loaded and fatty-acid-free preparations behave quite differently.
