Irradiated Trypsin 1:250 Dry Powder

This product is a dry powder trypsin preparation with 1:250 activity classification for cell culture-related dissociation and digestion reagent formulation. As an animal-origin protease, it is suitable for teams that require a conventional trypsin raw material rather than a pre-diluted ready-to-use solution. The material has been irradiated, is supplied as powder, and is packaged as 100 g under SKU CCM-RG-0052. The listed pack price is USD 2,197.92.

Key specifications include endotoxin ≤20 EU/mg, shelf life of 24 months, storage at 2–8 °C protected from light, and blue-ice shipping. pH and osmolality are not properties of the dry powder itself; they should be verified after reconstitution in the user’s selected buffer or balanced salt solution. This item is part of our cell culture reagents range for process development, R&D, and routine cell-culture support.

• Preparation of trypsin-based dissociation solutions for adherent mammalian cell passaging, scale-up studies, and routine culture maintenance.
• Formulation of custom digestion reagents where the laboratory controls concentration, buffer composition, exposure time, inhibitor system, pH, osmolality, and filtration approach.
• Raw-material qualification for research-scale and process-development workflows requiring documented endotoxin specification and defined cold-chain handling.
• Comparability studies between in-house trypsin formulations and existing dissociation protocols for cell line development, media screening, and assay-readiness workflows.
• Use as a component in controlled cell detachment procedures where operators optimize enzyme contact time to protect cell morphology, attachment recovery, and downstream culture performance.

Store unopened material at 2–8 °C and protect from light for the stated 24-month shelf life. Shipments are dispatched on blue ice to support temperature-controlled receipt. After opening, handle the powder using aseptic technique in a controlled laboratory environment, minimize moisture uptake, and reseal promptly. Reconstituted solutions should be prepared using validated laboratory procedures, checked for target pH and osmolality, and filtered or processed according to the user’s internal protocol. Avoid repeated warming of the bulk powder and document lot opening, reconstitution date, and working-solution storage conditions for traceability.

CellCultureMedia supplies this irradiated trypsin powder for research, cell culture, and bioprocessing workflows with specification-focused documentation for procurement and technical review. Manufacturing and release practices are ISO-aligned, and a Certificate of Analysis can be requested for lot-specific confirmation of identity, endotoxin specification, appearance, and release status. Visit our quality documentation page for documentation expectations, or use the request quote form for bulk requirements, scheduled supply, or institution-specific purchasing terms. Free worldwide shipping is available on standard qualified orders, with cold-chain packing matched to the product’s blue-ice shipping condition.