Animal Origin-Free Collagenase Type IV Powder

CCM-RG-0055 is Collagenase Type IV supplied in powder form for use as a dissociation reagent in cell culture workflows. Type IV collagenase is commonly selected when process teams need collagen-degrading activity with controlled handling of extracellular matrix structures. It is used during tissue mincing, matrix digestion, and preparation of cell suspensions where collagen content limits mechanical dissociation alone. The product is packaged as 100 mg and priced at $319.44, making it suitable for R&D-scale method development, pilot digestion studies, and qualification of repeatable enzymatic dissociation steps.

The material is classified as animal origin-free and falls under the cell culture reagents category, specifically dissociation reagents. As a dry powder, pH and osmolality are not intrinsic release specifications for the supplied format; users should define and verify pH, osmolality, buffer composition, calcium concentration, enzyme concentration, incubation temperature, and exposure time after reconstitution. For procurement review, request lot-specific documentation through a formal quote, including available release information and current lead time.

• Enzymatic dissociation of collagen-containing tissue fragments for research cell culture preparation.
• Processing of extracellular matrix-rich samples where mechanical disruption alone is insufficient or produces inconsistent yield.
• Development of primary cell isolation methods requiring a collagenase format compatible with defined buffer systems.
• Optimization of digestion parameters, including enzyme loading, incubation time, agitation method, and quench conditions.
• Use in R&D, academic, biotechnology, and bioprocess development laboratories evaluating dissociation reagent performance.
• Preparation of cell suspensions before downstream washing, filtration, counting, culture seeding, or analytical characterization.

Store the unopened powder at 2-8 °C protected from light. The stated shelf life is 24 months when the material is maintained under the recommended storage conditions and handled using appropriate laboratory controls. Shipments are dispatched on blue ice to support refrigerated transport. After receipt, transfer the container to qualified cold storage promptly and avoid repeated temperature excursions. Reconstitute only with buffers qualified for the intended cell culture process, and prepare aliquots if repeated access is expected. Because enzyme activity can be affected by buffer composition, divalent cations, pH, temperature, and hold time, users should qualify working solutions within their own protocol and discard unused solution according to internal procedures.

CellCultureMedia supplies CCM-RG-0055 for research, cell culture, and bioprocess development use, with procurement support for lot traceability and documentation review. Manufacturing and supply controls are managed using ISO-aligned quality practices, and a Certificate of Analysis is available on request when released for the applicable lot. Where sterility, endotoxin, or activity values are required for internal qualification, request the current lot documentation before purchase so your quality, process, or R&D team can confirm suitability. Additional support for specifications, documentation, and supplier qualification is available through the quality documentation channel. Free worldwide shipping is available for eligible orders, and bulk pricing can be quoted for recurring or scale-up demand.