ADCF Serum-Free CHO Feed Supplement Set

CCM-SF-0034 is designed for serum-free fed-batch supplementation where consistent nutrient delivery, animal component-free formulation status, and lot documentation are important procurement criteria. The set is supplied in liquid format as two coordinated components: a 100 mL feed and a 10 mL supplement. It is intended for use with established CHO host systems including CHO K1, CHO-S, CHO-GS, and CHO DG44, supporting R&D screening, shake flask optimization, and scale-up evaluation in bioprocess development.

The formulation is classified as animal origin-free and serum-free, helping facilities reduce variability associated with undefined serum inputs while maintaining compatibility with common chemically controlled production platforms. The recommended culture environment is 37 °C and 5–10% CO2, consistent with standard mammalian cell culture practice. As part of the CellCultureMedia serum-free media portfolio, this feed set is positioned for recombinant protein expression, biologics process development, and vaccine research manufacturing support where CHO cell performance and traceable supply are required.

• Fed-batch CHO process development: Use as a supplemental feed set during growth and production phases for CHO K1, CHO-S, CHO-GS, and CHO DG44 cultures when serum-free and animal component-free inputs are specified.
• Clone and media screening: Evaluate feed response across candidate clones, host lines, basal media platforms, and feeding schedules in shake flasks, deep-well plates, spinner cultures, or bench-scale bioreactors.
• Biopharmaceutical expression workflows: Supports cell culture operations for recombinant proteins, antibodies, and related biologics in research and process characterization settings.
• Serum-reduction and animal component-free conversion: Suitable for teams replacing serum-containing supplements with more defined liquid feed components, subject to internal qualification.
• Scale-up comparability studies: The liquid format simplifies volumetric dosing and feed timing studies from small-volume process development through pilot evaluation.
• Procurement-controlled supply: Available under CCM-SF-0034 for purchasing teams that need a defined pack size, documented storage conditions, published endotoxin specification, and quote-based ordering through CellCultureMedia request channels.

Store CCM-SF-0034 at 2–8 °C protected from light. Do not freeze unless a validated internal procedure has confirmed post-thaw performance for the intended process. The product is supplied sterile-filtered and should be handled aseptically after opening to preserve sterility during repeated use. Recommended transport is on blue ice; inspect the shipment on arrival, record the condition of the bottles, and transfer to refrigerated storage promptly. Shelf life is 12 months when the unopened product is stored as specified. Before use, equilibrate only the required volume to working temperature, mix gently without foaming, and avoid extended exposure to room temperature or direct light.

Each lot is manufactured under ISO-aligned quality practices for research and bioprocessing supply. The product is sterile-filtered, animal origin-free, serum-free, and specified for endotoxin at ≤20 EU/mL. A Certificate of Analysis can be requested for applicable lots, and additional documentation may be available for supplier qualification, incoming inspection, and process-change review. Visit CellCultureMedia quality resources for documentation expectations and support options. Free worldwide shipping is available where permitted by destination requirements, with blue-ice logistics used to maintain appropriate transport conditions for this refrigerated liquid supplement set.