Puromycin 10 mg/mL Cell Culture Reagent

CCM-RG-0039 is a 10 mg/mL puromycin solution supplied as a 1 mL liquid reagent for cell culture selection protocols. Puromycin is an aminonucleoside antibiotic commonly used to select mammalian or other eukaryotic cells carrying a compatible resistance marker. In culture systems, it acts rapidly by interfering with protein synthesis, enabling efficient enrichment of resistant cell populations when the working concentration has been established for the specific cell type, medium composition, and process design.

The product is classified as animal origin-free and is sterile-filtered for direct use as a concentrated stock in qualified laboratory workflows. Key specifications include liquid form, pH 6.0–7.0, endotoxin ≤5 EU/mL, and a declared shelf life of 24 months under recommended storage conditions. It is part of the CellCultureMedia cell culture reagents portfolio and is suitable for procurement programs that require defined documentation, cold-chain control, and lot-specific quality visibility.

• Selection of cultured cells engineered to express a validated puromycin resistance marker, following laboratory-established kill curve data.
• Maintenance of stable cell pools or clonal cell lines after the appropriate sustaining concentration has been determined for the cell model.
• Support for transfection, transduction, and genome-editing workflows where antibiotic selection is used as a process step.
• Use in research cell culture systems that require an animal origin-free antibiotic stock and controlled endotoxin specification.
• Scale-aware reagent sourcing for R&D groups, core facilities, and process development teams that need consistent 1 mL units or bulk purchasing through request-for-quote procurement.

Store CCM-RG-0039 at -30 to -5 °C protected from light. The product ships on dry ice to maintain frozen cold-chain conditions during transit. Upon receipt, verify that the vial remains frozen or appropriately cold, record the lot information, and transfer promptly to a qualified freezer. Avoid repeated freeze-thaw cycles by aliquoting under aseptic conditions if multiple uses are expected. Thaw only the quantity required for near-term work, mix gently after thawing, and do not use if visible contamination, unexpected turbidity, or container damage is observed. Always determine an application-specific working concentration before routine use, because puromycin sensitivity varies by cell type, culture density, serum level, exposure time, and expression system.

This puromycin reagent is manufactured and released using ISO-aligned quality practices appropriate for research and bioprocessing supply chains. Sterile filtration, animal origin-free classification, pH specification, endotoxin limit, and shelf-life assignment support controlled incoming material review by procurement, QA, and laboratory operations teams. A lot-specific Certificate of Analysis is available on request, and additional documentation can be coordinated through the CellCultureMedia quality support process. Free worldwide shipping is offered where available, with dry-ice packaging used for temperature-sensitive dispatch. For standing orders, multi-lot planning, or volume-based pricing, customers may request a formal quotation referencing CCM-RG-0039.