Phleomycin D1 Selective Antibiotic, 100 mg/mL

Phleomycin D1 selective antibiotic is used as a cell culture reagent for selection of cells carrying compatible resistance markers. CCM-RG-0029 is supplied as a liquid concentrate at 100 mg/mL in a 1 mL pack size, enabling dilution into complete culture medium according to validated internal protocols. The product is classified as animal-origin-free and sterile-filtered, making it suitable for research and process-development environments where component traceability and contamination control are procurement priorities.

Key release specifications include pH 7.1-7.7, endotoxin at ≤1 EU/mL, sterile-filtered status, and a 24-month shelf life when stored as directed. The reagent is categorized under antibiotics and antimycotics within cell culture reagents. It is commonly evaluated during transfection, stable pool generation, clone selection, and maintenance of engineered mammalian or other eukaryotic culture systems. Users should determine the working concentration, exposure schedule, and medium compatibility for each cell type, passage range, and culture format.

• Selection of transfected or transduced cell populations carrying an appropriate resistance cassette in plate, flask, or bioreactor-development formats.
• Maintenance of selection pressure during expansion of stable pools, clonal isolates, or engineered research cell banks after kill-curve qualification.
• Screening workflows where a compact, high-concentration 100 mg/mL stock supports multiple dosing levels from a single 1 mL vial.
• R&D and process-development studies requiring animal-origin-free reagent classification, sterile-filtered supply, low endotoxin specification, and documented shelf life.
• Media supplementation studies in which antibiotic concentration, culture duration, viability, expression stability, and passage history are tracked as controlled process variables.

Store CCM-RG-0029 at -30 to -5 °C and keep the container protected from light. The product ships on dry ice to preserve frozen temperature conditions during transit; on receipt, confirm that the vial remains frozen or cold and transfer it promptly to an appropriate freezer. Avoid repeated freeze-thaw cycles by aliquoting under aseptic conditions if your workflow requires multiple uses. Thaw gently, mix by inversion rather than vigorous agitation, and add to culture medium only after the concentrate is fully homogeneous. Follow institutional handling procedures for selective antibiotics, including suitable personal protective equipment and disposal practices for supplemented media.

CellCultureMedia supplies CCM-RG-0029 for research and bioprocessing workflows under ISO-aligned supplier controls, with specification review focused on sterility, endotoxin, pH range, animal-origin-free classification, shelf life, storage, and shipping conditions. A Certificate of Analysis can be requested for lot-specific documentation through the quality documentation channel. For planned campaigns, multi-vial requirements, or standing-order programs, procurement teams can use the request a quote workflow to confirm pricing, availability, shipping schedule, and any applicable free worldwide shipping options before purchase order placement.