PBS-EDTA Solution, Calcium and Magnesium Free

CCM-RG-0009 is a PBS-EDTA balanced salt solution designed for routine cell culture reagent use in research, development, and bioprocess support laboratories. The formulation includes 1 mM EDTA and excludes phenol red, calcium ions, and magnesium ions. The absence of divalent cations helps reduce cation-dependent cell adhesion during wash steps and supports controlled handling before enzymatic or non-enzymatic dissociation. The phenol red-free composition is useful when visual color indicators are not desired or when downstream optical measurements require a cleaner background.

This product is supplied as a sterile-filtered liquid in a 500 mL format. Key specifications include pH 7.1 to 7.3, osmolality 280 to 320 mOsmol/kg, endotoxin level ≤1 EU/mL, animal-origin-free classification, and a stated shelf life of 48 months when stored as directed. For related buffers, dissociation aids, and washing solutions, procurement teams can review the broader cell culture reagents category. The product is intended for cell culture, R&D, assay preparation, and process development environments where consistent buffer composition and low bioburden risk are required.

• Washing adherent or suspension cell cultures before passaging, medium exchange, or harvest, especially where calcium and magnesium removal is required.
• Preparing cells for dissociation workflows by reducing divalent-cation-mediated adhesion before the addition of compatible dissociation reagents.
• Rinsing culture vessels, microcarriers, and cell pellets in research or process development workflows that require a phenol red-free balanced salt solution.
• Supporting assay setup where background color from phenol red may interfere with absorbance, fluorescence, imaging, or visual inspection requirements.
• Use in animal-origin-free reagent systems for laboratories documenting material origin, batch consistency, and controlled input specifications.
• Buffering cell handling steps in academic, biotech, and biopharma laboratories that require defined pH range, osmolality range, sterility status, and endotoxin specification.

Store CCM-RG-0009 at 2 to 30 °C protected from light. The solution is shipped at room temperature, and the receiving site should place the bottle into approved storage conditions upon receipt. Use aseptic technique when opening and dispensing to preserve sterility over the in-use period. Do not use the product if the container is compromised, if turbidity or visible particulates are observed, or if the solution has been stored outside the validated range for an extended period. For multi-bottle procurement, rotate stock by expiry date and document lot numbers in batch records or laboratory notebooks. The stated shelf life is 48 months under specified storage conditions.

CellCultureMedia supplies CCM-RG-0009 for organizations that require specification-based reagent purchasing and traceable quality documentation. Manufacturing and release processes are ISO-aligned, with sterile filtration, endotoxin specification, pH range, osmolality range, storage conditions, shipping conditions, and shelf-life attributes defined for procurement review. A Certificate of Analysis is available on request, and additional documentation can be coordinated through the quality documentation team. Standard orders include free worldwide shipping, while larger recurring requirements can be quoted through request a quote to align volume, delivery schedule, and documentation needs.