N-2 Serum-Free Supplement 100X

CCM-RG-0065 is a 100X N-2 serum-free supplement supplied as a liquid additive for use in defined cell culture media. It is specified as animal-origin-free and sterile filtered, supporting laboratories that need reduced raw-material complexity and consistent sourcing for research, assay development, and bioprocess support. The product is provided in a 5 mL pack size at a listed price of $402.78, suitable for evaluation lots, small-batch media preparation, or qualification before larger procurement.

Key release specifications include pH 6.0 to 10.0, osmolality 30 to 60 mOsmol/kg, endotoxin not more than 2 EU/mL, and a 24-month shelf life when stored as directed. The low-osmolality additive format allows users to incorporate the supplement into compatible basal formulations without materially shifting final medium osmolality when diluted at the intended 1X working concentration. For related defined supplements and media components, see our cell culture reagents category.

• Preparation of serum-free culture media for neuronal, neural progenitor, and other specialized mammalian cell models requiring N-2-type supplementation.
• Defined-media development where animal-origin-free input materials are preferred for traceability, risk assessment, and simplified vendor qualification.
• Cell and gene processing research, including media optimization studies, cell expansion feasibility work, and controlled culture condition screening.
• R&D studies comparing serum-containing and serum-free culture systems, especially where variability from serum lots needs to be minimized.
• Process engineering evaluations that require documented sterility, endotoxin, pH, osmolality, shelf-life, and shipping conditions for incoming material review.

Store CCM-RG-0065 at -30 to -5 °C protected from light. The product is shipped on dry ice to maintain frozen temperature control during transit. Upon receipt, confirm that the material remains frozen, record the lot number, and place the vial or bottle into controlled frozen storage immediately. Avoid repeated freeze-thaw cycles; if routine use requires multiple openings, thaw under controlled laboratory conditions and aliquot aseptically into sterile, compatible containers. Use only in validated cell culture protocols and add to medium using aseptic technique. The assigned shelf life is 24 months when stored unopened under the recommended conditions.

CellCultureMedia supplies CCM-RG-0065 for research and bioprocessing workflows that require specification-driven purchasing. Manufacturing and quality release are managed under ISO-aligned quality practices, with sterile filtration and routine release testing for critical parameters including endotoxin, pH, osmolality, appearance, and shelf-life assignment. A Certificate of Analysis is available on request for lot review, incoming QC, and vendor file maintenance; visit quality documentation for more information. For volume demand, recurring supply, or project pricing, submit requirements through request a quote. Free worldwide shipping is available for qualified orders, with dry-ice logistics used where temperature control is required.