MDCK-S ADCF Serum-Free Medium Powder

CCM-BP-0003 is supplied as a dry powder medium for preparation of 50 L of serum-free, animal-origin-free medium for MDCK-S suspension culture. It is intended for bioprocess media workflows where defined composition, lot traceability, and scalable preparation are important to process development and manufacturing support teams. The formulation contains phenol red for visual pH indication, D-glucose as the principal carbohydrate, 4 mM L-glutamine, and 2 mM L-alanyl-L-glutamine to support nitrogen availability and improve formulation robustness during storage and preparation.

Key release specifications include osmolality of 240–280 mOsmol/kg, pH of 3.5–4.5 as specified for the supplied product, endotoxin not more than 3 EU/mL, and a 24-month shelf life when stored as directed. The medium is classified as animal-origin-free and serum-free, making it suitable for organizations standardizing inputs across suspension MDCK processes. For related formats and procurement comparisons, visit the bioprocess media category or request a project-specific quotation.

• Suspension MDCK-S cell culture in serum-free, animal-origin-free process development.
• Upstream bioprocess studies requiring a powdered medium format for larger-volume preparation and inventory efficiency.
• Seed train, process characterization, and scale-up experiments operated at 37 °C with 5–10% CO2.
• Biopharmaceutical production research where defined raw materials, endotoxin limits, and lot documentation are procurement requirements.
• Media screening and comparability studies for teams evaluating powder-to-liquid preparation, osmolality targets, and amino acid supplementation strategies.

The 50 L presentation is appropriate for laboratories and pilot groups that require more than small screening volumes but still need controlled ordering, documentation review, and manageable storage. Process engineers should confirm compatibility with the intended hydration procedure, filtration approach, supplements, vessel configuration, and cell banking strategy before implementation.

Store the unopened powder at 2–8 °C protected from light. The product is shipped on blue ice to support temperature-controlled logistics during transit; inspect packaging upon receipt and transfer promptly to refrigerated storage. Use aseptic technique during reconstitution and downstream handling, and prepare only with qualified water, mixing equipment, pH adjustment materials, and filtration methods validated by the user. Avoid repeated warming of the dry powder container, minimize moisture exposure, and close the container immediately after dispensing. When prepared as liquid medium, establish internal hold-time, sterility, pH, and osmolality acceptance criteria appropriate to the local process.

CellCultureMedia supplies CCM-BP-0003 for research and bioprocessing organizations that require specification-driven purchasing, lot control, and responsive documentation. Manufacturing and quality release are managed in an ISO-aligned quality environment, with lot-specific Certificate of Analysis availability on request through the quality documentation team. Typical documentation can support incoming inspection, raw material qualification, and change-control review, including the stated pH, osmolality, endotoxin, shelf-life, form, storage, and shipping conditions.

The listed 50 L pack is priced at $15,080.56, and free worldwide shipping is available where import and cold-chain routing can be supported. For volume commitments, standing orders, or multi-site supply planning, submit requirements through request a quote so the team can confirm availability, lead time, freight lane, and documentation needs before purchase order placement.