BHK-21 Serum-Free ADCF Medium

This BHK-21 serum-free medium is designed for laboratories and process groups that require a defined, animal-origin-free formulation for baby hamster kidney cell culture. It is supplied as a sterile-filtered liquid and specified as serum-free, supporting users who want to reduce lot-to-lot variability associated with serum-containing systems. The formulation includes phenol red for visual pH indication and is intended for controlled CO2 incubator operation at 37 °C with 5-10% CO2. For related defined media formats, see the serum-free media category.

Key release specifications include pH 7.0-7.4, osmolality 270-340 mOsmol/kg, endotoxin ≤3 EU/mL, sterile-filtered processing, and a stated shelf life of 24 months when stored correctly. The product is configured for BHK-21 applications in research and bioprocess development where animal-derived component-free sourcing, documentation, and reproducible handling are important. This configuration is supplied without L-glutamine and sodium bicarbonate, allowing process teams to set those supplements according to their validated platform, CO2 range, and feeding strategy.

• Serum-free culture of BHK-21 cells in research, scale-up, and process development studies.
• Animal-derived component-free culture workflows where reduced undefined input is required for comparability and documentation.
• Evaluation of BHK-21 growth, maintenance, and productivity in controlled 37 °C, 5-10% CO2 incubator conditions.
• Adaptation studies from serum-containing or lower-serum media into chemically defined culture conditions.
• Screening of supplementation strategies, including user-defined L-glutamine and bicarbonate levels for platform-specific pH control.
• Biopharmaceutical and viral production research workflows that use BHK-21 host cells under documented, serum-free conditions.

Store CCM-SF-0043 at 2-8 °C protected from light. Do not freeze unless a validated internal procedure confirms acceptable performance after freeze-thaw exposure. Warm only the required volume to working temperature and use aseptic technique when transferring medium to culture vessels or feed containers. Because this format is supplied without L-glutamine and sodium bicarbonate, add those components only according to the laboratory’s qualified protocol and mix thoroughly before use. Inspect the liquid before dispensing; do not use if the container is compromised, the medium is turbid, or unexpected particulates are observed.

CellCultureMedia supplies CCM-SF-0043 for research and bioprocessing procurement programs that require traceable specifications and consistent logistics. Manufacturing and release controls are ISO-aligned, with product testing focused on sterility, endotoxin, pH, osmolality, shelf life, and lot identification. A Certificate of Analysis is available on request through the quality documentation channel, and procurement teams can request a quote for standing orders, multi-bottle purchases, or scheduled replenishment. The 500 mL bottle ships on blue ice, and eligible orders include free worldwide shipping to streamline international sourcing.