Hygromycin B 50 mg/mL Sterile Cell Culture Reagent

CCM-RG-0031 is Hygromycin B at 50 mg/mL in liquid format, manufactured as a sterile-filtered, animal origin-free cell culture reagent. Hygromycin B is an aminoglycoside antibiotic commonly used as a selection pressure in cultures containing a compatible resistance marker. The 50 mg/mL concentration supports accurate dilution into complete media, enabling laboratories to prepare working concentrations according to validated cell-line-specific kill curve data and internal protocols.

This 10 mL unit is classified under antibiotics and antimycotics within cell culture reagents. It is suitable for laboratories that require a defined, convenient selection reagent without animal-derived components. Source specifications identify the product as a sterile liquid with 24-month shelf life when stored as directed. pH and osmolality are not listed for this item; users should qualify final culture medium performance after supplementation when these parameters are process-critical.

• Selection and maintenance of mammalian cell populations carrying hygromycin-resistance marker constructs in research and process-development culture systems.
• Preparation of antibiotic-supplemented complete media for stable cell line generation workflows, following laboratory-established working concentrations.
• Use in screening workflows where Hygromycin B sensitivity has been established by kill curve testing for the target host cell line.
• Support for suspension or adherent culture processes where a sterile-filtered, animal origin-free antibiotic reagent is specified by the bill of materials.
• Procurement-controlled reagent standardization for academic, biotechnology, and biopharma laboratories using lot-tracked antibiotic selection materials.

Store Hygromycin B 50 mg/mL at 2–8 °C protected from light. Do not leave the bottle at room temperature for extended periods during media preparation, and return unused material to refrigerated storage promptly after aseptic dispensing. The product is shipped on blue ice to support refrigerated logistics during transit. Shelf life is 24 months when the container is stored unopened under the specified conditions. For best practice, record the receipt date, lot number, opening date, and number of freeze-warm or temperature excursion events if applicable under your internal quality system.

CellCultureMedia supplies CCM-RG-0031 for professional cell culture use with sterility status, origin classification, storage requirements, and shelf-life information aligned to procurement documentation needs. Manufacturing and release controls are organized under ISO-aligned quality practices, and lot-specific documentation such as a Certificate of Analysis can be requested through the quality documentation channel. The listed 10 mL unit price is USD 777.78, and free worldwide shipping is included where available. For standing orders, multi-unit purchasing, or site-wide supply agreements, submit requirements through request a quote so our team can confirm lead time, freight route, and volume pricing.