HBSS with Calcium, Magnesium and Bicarbonate
HBSS with sodium bicarbonate, calcium, and magnesium is a sterile, animal-origin-free balanced salt solution supplied without phenol red for cell culture handling and short-term cell maintenance. This 500 mL liquid reagent is formulated with D-glucose, bicarbonate buffering components, Ca2+, and Mg2+ for routine laboratory workflows where a defined salt environment is required. CellCultureMedia supplies CCM-RG-0011 at $47.22 with free worldwide shipping, with quotation support available through request a quote for multi-unit or recurring procurement.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-RG-0011 is Hank’s Balanced Salt Solution supplied as a ready-to-use liquid reagent for cell culture-related processing. The formulation contains 1.0 g/L D-glucose, 0.35 g/L sodium bicarbonate, calcium ions, and magnesium ions, and it is manufactured without phenol red. The absence of phenol red is useful where optical interference, color contribution, or assay background from indicator dye is not desired. As a balanced salt solution, it provides inorganic salts, glucose, and buffering support for washing, rinsing, dilution, and interim cell handling steps outside complete growth medium.
The product is classified as animal-origin-free and filter sterilized. Key release specifications include pH 7.0–7.6, osmolality 260–300 mOsmol/kg, and endotoxin not more than 1 EU/mL. The liquid format reduces preparation variability compared with in-house powder reconstitution and supports consistent use across R&D, process development, and academic laboratory environments. Procurement teams can source this reagent within the broader cell culture reagents portfolio for harmonized purchasing and documentation workflows.
Applications
- Washing adherent or suspension cells during medium exchange, subculture preparation, or sample processing when a balanced isotonic salt environment is required.
- Short-term holding and manipulation of mammalian cells during routine culture operations where calcium and magnesium are compatible with the workflow.
- Rinsing culture vessels, cell layers, or harvested cell material before downstream analytical preparation.
- Dilution or transfer steps in cell culture-related reagent workflows requiring HBSS without phenol red.
- Assay preparation where removal of phenol red helps reduce background color contribution in optical measurements.
- Process development studies that require a defined, sterile, animal-origin-free balanced salt solution with documented pH, osmolality, endotoxin, and shelf-life attributes.
Storage and Handling
Store CCM-RG-0011 at 2–30 °C protected from light. The product ships at ambient temperature and has a stated shelf life of 24 months when stored unopened under recommended conditions. Inspect the bottle before use and do not use if the container is compromised, visibly contaminated, or outside internal acceptance criteria. Use aseptic technique after opening to help maintain sterility, and minimize repeated warming, prolonged light exposure, and unnecessary handling. Because this formulation contains calcium and magnesium, confirm compatibility with dissociation reagents, chelators, and cell-specific protocols before implementation in a validated workflow.
Quality and Documentation
This HBSS is produced under ISO-aligned quality practices for research and bioprocessing supply chains, with filtration-based sterility control and release specifications covering pH, osmolality, endotoxin, format, shelf life, and storage conditions. A Certificate of Analysis is available on request through quality documentation support. CellCultureMedia provides free worldwide shipping for this 500 mL unit and can support consolidated ordering for laboratories standardizing multiple balanced salt solutions, sera, media, and reagents across purchasing locations.
Specifications
Formulation Highlights
Contains (+)
1.0 g/L D-glucose, 0.35 g/L sodium bicarbonate, Ca2+, Mg2+
Does Not Contain (-)
Phenol red
Storage & Handling
Storage: 2~30 ℃, protected from light. Shelf life: 24 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
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Learn more about this
- HBSS (Hank's Balanced Salt Solution)HBSS (Hank's Balanced Salt Solution) is an isotonic balanced salt solution used to wash cells, transport tissue, dilute reagents and hold cells briefly outside their growth medium. It contains 8.0 g/L sodium chloride, 1.0 g/L D-glucose, phosphate and 350 mg/L sodium bicarbonate, and is supplied either with calcium and magnesium (1.26 mM Ca, ~0.9 mM Mg total) or without them. Its low bicarbonate means it is designed for use at atmospheric CO2 or in sealed vessels, not for prolonged culture in a 5% CO2 incubator.
- PBS vs DPBS: What Is Different, and Which One to UsePBS and DPBS are both phosphate-buffered saline solutions; the difference is the recipe, not the function. Dulbecco's formulation (DPBS) adds potassium chloride and carries roughly twice the phosphate of a typical PBS (about 9.5 mM versus about 4 mM), and it is sold in two versions - with calcium and magnesium, and without. In practice the with-or-without-divalent-cations choice matters far more than the PBS-or-DPBS label: use a calcium- and magnesium-free solution to wash cells before trypsinisation or EDTA dissociation, because Ca2+ and Mg2+ support the cadherin and integrin bonds you are about to break, and use the version containing calcium and magnesium when cells must stay attached and intact through the wash.
- Cell culture buffersCell culture buffers hold medium in the pH 7.2-7.4 range that mammalian cells require, against the acid load cells generate as they metabolise. The default system in almost every classical medium is sodium bicarbonate working with the CO2 in the incubator atmosphere, which is why bicarbonate content must be matched to the incubator setting: about 1.5-2.2 g/L NaHCO3 for 5% CO2 and 3.7 g/L for 10% CO2. Organic buffers such as HEPES (pKa 7.48) are added at 10-25 mM to hold pH when cultures are outside a CO2 atmosphere, while phosphate-buffered solutions such as PBS and DPBS are used for washing and short-term handling rather than for growth.
- DispaseDispase, also sold as Dispase II or neutral protease, is a zinc-dependent metalloprotease from Paenibacillus (formerly Bacillus) polymyxa, EC 3.4.24.4, used for gentle tissue dissociation and for detaching cells and epithelial sheets intact. It cleaves fibronectin and type IV collagen, degrades type I collagen only minimally, and does not cleave laminin or type V collagen, which is why it can separate an epidermis from a dermis or lift a confluent epithelial sheet off plastic without destroying cell-cell junctions. Because it is a metalloprotease requiring zinc for catalysis and calcium for stability, it is inhibited by EDTA, EGTA and 1,10-phenanthroline, and it must be diluted in a calcium-containing buffer rather than a chelator-based one.
