HBSS with Phenol Red, without Calcium and Magnesium
HBSS with phenol red, without calcium and magnesium, is a sterile balanced salt solution for rinsing, dilution, and short-term maintenance of cultured cells. CCM-RG-0012 is supplied as a 500 mL animal origin-free liquid formulated with D-glucose and sodium bicarbonate, with phenol red as a pH indicator. The product is sterile-filtered, specified at pH 7.0 to 7.4 and osmolality 260 to 300 mOsmol/kg, and ships at room temperature with free worldwide shipping available for eligible orders.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-RG-0012 is a ready-to-use Hank's Balanced Salt Solution for cell culture workflows that require an isotonic salt matrix without divalent calcium or magnesium ions. The formulation contains phenol red for visual pH monitoring, 1.0 g/L D-glucose as an energy source, and 0.35 g/L sodium bicarbonate for buffering in CO2-compatible handling environments. The absence of Ca2+ and Mg2+ is useful when protocols require reduced cell-cell or cell-surface adhesion during washing steps, reagent dilution, or preparation before dissociation procedures. For related buffers, rinses, and liquid handling reagents, see our cell culture reagents category.
The product is supplied as a liquid in a 500 mL bottle and is classified as animal origin-free. Key release specifications include sterile-filtered manufacturing, endotoxin not more than 1 EU/mL, pH 7.0 to 7.4, and osmolality 260 to 300 mOsmol/kg. This specification profile supports procurement teams that need consistent lot documentation for research, assay development, and upstream process support materials. The listed unit price is $47.22 for 500 mL, with quote support available for standing orders, multi-bottle purchases, and laboratory supply consolidation.
Applications
- Rinsing adherent mammalian cell monolayers before media exchange, passaging, transfection setup, or analytical sampling when calcium and magnesium are not required by the protocol.
- Dilution or preparation of cell culture reagents where an isotonic, buffered salt solution with phenol red is preferred for visual pH status checks during bench handling.
- Short-duration holding, washing, or transfer of suspension cells in research and bioprocess development workflows, provided the exposure time and temperature are validated by the user.
- Pre-dissociation washing steps before use of separately sourced detachment reagents, where removal of serum proteins and exclusion of Ca2+ and Mg2+ can improve procedural consistency.
- Assay preparation workflows that require a defined balanced salt solution containing D-glucose and sodium bicarbonate but excluding added calcium chloride and magnesium salts.
Storage and Handling
Store CCM-RG-0012 at 2 to 30 °C and protect the bottle from light to preserve phenol red performance and formulation integrity. The product ships at room temperature and does not require refrigerated freight under the stated shipping condition. Shelf life is 24 months when stored unopened within the recommended temperature range. Use aseptic technique after opening, keep the cap closed when not dispensing, and avoid repeated exposure to non-sterile pipettes, tubing, or reservoirs. Do not use material that shows visible contamination, turbidity, precipitate inconsistent with normal appearance, damaged packaging, or a color shift outside the expected pH indicator range for the intended workflow.
Quality and Documentation
CellCultureMedia supplies CCM-RG-0012 for research, development, and bioprocessing support under ISO-aligned manufacturing and quality control practices. Lot-level documentation can be requested through our quality team, including Certificate of Analysis availability with core release attributes such as sterility, endotoxin, pH, osmolality, shelf life, and appearance where applicable. Procurement managers can use the CCM SKU for ordering, receiving, and inventory control across single-site or multi-site programs.
For project pricing, recurring supply, or larger-volume purchasing, submit requirements through request a quote. Include required pack count, delivery country, target arrival date, documentation needs, and any internal vendor qualification forms. Free worldwide shipping is available for eligible orders, and standard room-temperature dispatch helps simplify logistics compared with cold-chain materials. CellCultureMedia can also support cross-referencing against internal material descriptions when teams are standardizing balanced salt solution supply across R&D, pilot, or process engineering groups.
Specifications
Formulation Highlights
Contains (+)
[+]Phenol Red[+]1.0 g/L D-Glucose[+]0.35 g/L Sodium Bicarbonate
Does Not Contain (-)
[-]Ca 2+ [-]Mg 2+
Storage & Handling
Storage: 2~30 ℃, protected from light. Shelf life: 24 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
About this product
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Learn more about this
- HEPES buffer in cell cultureHEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic buffer used in cell culture to hold medium at physiological pH without depending on incubator CO2. Its pKa is about 7.5 at 20-25 degrees C and about 7.3 at 37 degrees C, giving useful buffering across roughly pH 6.8-8.2, and it is normally added to medium at 10-25 mM from a sterile 1 M stock. Because HEPES buffering does not rely on the carbonic acid/bicarbonate equilibrium, HEPES-supplemented medium resists the fast alkaline drift that occurs when a flask leaves a 5% CO2 incubator.
- HBSS (Hank's Balanced Salt Solution)HBSS (Hank's Balanced Salt Solution) is an isotonic balanced salt solution used to wash cells, transport tissue, dilute reagents and hold cells briefly outside their growth medium. It contains 8.0 g/L sodium chloride, 1.0 g/L D-glucose, phosphate and 350 mg/L sodium bicarbonate, and is supplied either with calcium and magnesium (1.26 mM Ca, ~0.9 mM Mg total) or without them. Its low bicarbonate means it is designed for use at atmospheric CO2 or in sealed vessels, not for prolonged culture in a 5% CO2 incubator.
- Phenol Red in Cell Culture MediaPhenol red (phenolsulfonphthalein) is a pH indicator added to cell culture media at roughly 5-15 mg/L, where it turns yellow below about pH 6.8 and pink to fuchsia above about pH 8.2, with the normal red-orange of healthy medium sitting near pH 7.4. Medium turning yellow means acidification - almost always lactate from cell metabolism, from an overgrown culture or from bacterial contamination - while medium turning pink or purple means the medium has gone alkaline, nearly always because CO2 has escaped from the bicarbonate buffer. Phenol red does no buffering itself and is purely diagnostic; use phenol-red-free medium for fluorescence and absorbance assays, and for oestrogen-responsive cell work, where the dye interferes with the readout.
