HBSS Balanced Salt Solution, Ca/Mg-Free, No Phenol Red

CCM-RG-0013 is a liquid HBSS balanced salt solution formulated for cell culture workflows requiring a calcium-free, magnesium-free, and phenol-red-free reagent. The formulation includes 1.0 g/L D-glucose as an energy substrate and 0.35 g/L sodium bicarbonate for bicarbonate buffering compatibility. It is designed as a balanced salt solution for short-term cell handling, washing, dilution, and preparation steps where ionic strength and osmolality control are required without adding divalent cations or visual pH indicator dye.

The solution is animal-origin-free, supplied as a 500 mL bottle, and manufactured as a sterile-filtered reagent. Core specifications include pH 7.0-7.6, osmolality 260-300 mOsmol/kg, endotoxin level ≤1 EU/mL, and a stated shelf life of 24 months when stored under recommended conditions. For laboratories standardizing reagent sourcing, this product belongs in the cell culture reagents category and is suitable for procurement as a defined, ready-to-use liquid salt solution. The absence of phenol red can help reduce optical or colorimetric background in imaging, plate reading, and assay-adjacent handling steps. The absence of Ca2+ and Mg2+ is useful when protocols require reduced cell adhesion support or when subsequent reagents are sensitive to divalent ions.

• Cell washing steps before medium exchange, passaging, counting, or assay setup where a balanced ionic environment is required.
• Short-term handling of adherent or suspension cell cultures during R&D workflows, media preparation, and process-development sampling.
• Preparation and dilution of cell suspensions when phenol red, calcium, and magnesium are not desired in the intermediate solution.
• Use in protocols that specify HBSS containing sodium bicarbonate and glucose, with no added Ca2+, Mg2+, or phenol red.
• Support for microscopy, plate-reader, and analytical workflows where reduced indicator dye background is preferred.
• Routine reagent stocking for academic, biotech, and biopharma laboratories that require defined liquid balanced salt solutions with documented pH, osmolality, sterility, endotoxin, and shelf-life specifications.

Compatibility depends on the cell type, exposure time, temperature, and complete protocol design. Process engineers and R&D teams should confirm suitability using internal qualification criteria before implementing this reagent in controlled workflows.

Store CCM-RG-0013 at 2-30 °C protected from light. The product is shipped at ambient temperature and should be transferred to controlled storage after receipt in accordance with internal laboratory procedures. Do not use if the container is damaged, leaking, visibly contaminated, or outside the labeled shelf-life period. Aseptic technique is recommended after opening to maintain reagent integrity through routine use. Avoid repeated unnecessary warming cycles and keep the bottle closed when not dispensing. The stated shelf life is 24 months under recommended storage conditions. For lot-specific handling questions or quotation support, contact CellCultureMedia through request a quote with the CCM SKU and required pack quantity.

This reagent is supplied as a sterile-filtered, animal-origin-free liquid balanced salt solution with specification targets for pH, osmolality, endotoxin, sterility, composition, and shelf life. Manufacturing and release controls are managed under ISO-aligned quality practices appropriate for research and bioprocessing supply chains. A lot-specific Certificate of Analysis is available on request for purchasing records, incoming QC, and internal material qualification. Review the CellCultureMedia quality documentation page for information on documentation workflows. Free worldwide shipping is available where logistics conditions permit, and bulk pricing can be quoted for recurring orders, multi-site sourcing, or consolidated reagent procurement programs.