G418 Selective Antibiotic 50 mg/mL
G418 Selective Antibiotic, 50 mg/mL, is a sterile-filtered liquid aminoglycoside reagent for selecting and maintaining transfected mammalian cell cultures carrying compatible resistance markers. Supplied as a 10 mL, animal-origin-free solution under SKU CCM-RG-0030, it supports research and process-development workflows that require reproducible selective pressure. The product is stored frozen at -30 to -5 °C, protected from light, and ships on dry ice with free worldwide shipping where service is available.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-RG-0030 is a ready-to-use 50 mg/mL G418 selective antibiotic solution supplied for cell culture reagent workflows. G418 is commonly used to select eukaryotic cells that express a compatible aminoglycoside resistance marker after plasmid, viral-vector, or other gene-delivery steps. The liquid format reduces weighing and dissolution steps, helps limit operator-to-operator variability, and is appropriate for laboratories that need consistent preparation of selection media across multiple passages or project teams.
This reagent is supplied in a 10 mL pack size and classified as animal-origin-free, supporting procurement requirements for defined raw-material sourcing. The solution is filter sterilized and intended for aseptic addition to complete culture media at user-qualified working concentrations. Source specifications include liquid form, sterile filtration, 24-month shelf life, frozen light-protected storage, and dry-ice shipment. pH and osmolality are not listed as release specifications for this product; users should qualify final medium parameters after dilution in the relevant formulation. For related antibiotics, buffers, and supplements, see our cell culture reagents category.
Applications
- Selection of mammalian cell populations expressing a compatible G418 resistance marker following stable transfection or other gene-introduction workflows.
- Maintenance of selective pressure in established recombinant cell lines during research-scale expansion, banking studies, clone screening, or process-development experiments.
- Support for comparative studies where a sterile, animal-origin-free liquid antibiotic is preferred to reduce preparation time and improve lot-to-lot handling consistency.
- Use in academic, biotechnology, and biopharma laboratories developing recombinant protein expression, reporter-cell systems, genome-engineering readouts, or cell-line characterization assays.
- Preparation of selective complete media after user-defined kill-curve studies. Effective working concentrations vary by cell type, medium composition, cell density, and exposure schedule, so each laboratory should establish selection and maintenance ranges under its own conditions.
Storage and Handling
Store CCM-RG-0030 at -30 to -5 °C and protect from light. The stated shelf life is 24 months when the container is stored under recommended conditions and handled using aseptic technique. Shipment is on dry ice to maintain frozen temperature control in transit. Upon receipt, verify that the material remains frozen or suitably chilled, transfer promptly to qualified frozen storage, and avoid repeated freeze-thaw cycles by aliquoting when appropriate. Thaw only the amount required for immediate use, mix gently after thawing, and do not use if visible contamination, leakage, or unexpected precipitation is observed. Because G418 activity can vary by cell system, final-use concentrations should be validated internally before routine adoption.
Quality and Documentation
CellCultureMedia supplies CCM-RG-0030 for research, development, and bioprocessing support under ISO-aligned quality practices focused on traceability, controlled handling, and specification review. Each order can be supported with lot-specific documentation, and a Certificate of Analysis is available on request through our quality team. Procurement teams may request current availability, bulk pricing, documentation, and shipping options through the request quote page. The list price for the 10 mL pack is $241.67, with free worldwide shipping available for eligible destinations. For larger programs, CellCultureMedia can coordinate recurring supply, consolidated shipments, and quotation support for multi-site purchasing.
Specifications
Storage & Handling
Storage: -30~-5 ℃, protected from light. Shelf life: 24 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
About this product
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Learn more about this
- Puromycin for Mammalian Cell SelectionPuromycin is an aminonucleoside antibiotic from Streptomyces alboniger that mimics the aminoacyl end of tRNA, enters the ribosomal A site and causes premature release of a truncated peptide chain, killing cells that cannot inactivate it. In mammalian cell culture it is used as a selection agent at 0.5-10 ug/mL, most commonly 1-2 ug/mL, and it kills non-resistant cells faster than any other common selection antibiotic -- typically within 2-5 days. Resistance is conferred by the pac gene encoding puromycin N-acetyltransferase, which acetylates the drug and renders it inactive. Because the effective concentration depends strongly on cell line, cell density and serum content, a kill curve must be run for each new cell line and each new lot of puromycin.
- Penicillin-Streptomycin and Antibiotic-Antimycotic in Cell CulturePenicillin-streptomycin, universally shortened to pen-strep, is supplied as a 100X sterile solution containing 10,000 units/mL penicillin G and 10,000 ug/mL streptomycin, diluted 1:100 into medium to give a working concentration of 100 U/mL penicillin and 100 ug/mL streptomycin. Penicillin blocks bacterial cell wall synthesis and covers mainly Gram-positive organisms; streptomycin binds the bacterial 30S ribosomal subunit and covers mainly Gram-negatives, so the pair gives broad antibacterial coverage. Antibiotic-antimycotic (anti-anti) is the same combination plus 25 ug/mL amphotericin B at 100X, giving 0.25 ug/mL in use for antifungal coverage. Neither controls mycoplasma, and major cell banks including ATCC recommend against routine antibiotic use because it masks low-level contamination rather than preventing it.
- Selection Antibiotics for Mammalian Cell LinesSelection antibiotics are cytotoxic agents used to isolate mammalian cells that carry a co-transfected resistance gene, by killing every cell that does not. The five standard agents are G418/geneticin (100–2000 µg/mL, resistance gene neo), hygromycin B (50–1000 µg/mL, hph), blasticidin S (1–20 µg/mL, bsd or bsr), zeocin (50–1000 µg/mL, Sh ble) and puromycin (0.5–10 µg/mL, pac). The correct concentration is not the one on the datasheet but the one determined empirically for your cell line by a kill curve — the lowest concentration that kills 100% of untransfected cells within the agent's normal selection window, which is 2–4 days for puromycin and up to 14 days for G418.
