G418 Selective Antibiotic 50 mg/mL

CCM-RG-0030 is a ready-to-use 50 mg/mL G418 selective antibiotic solution supplied for cell culture reagent workflows. G418 is commonly used to select eukaryotic cells that express a compatible aminoglycoside resistance marker after plasmid, viral-vector, or other gene-delivery steps. The liquid format reduces weighing and dissolution steps, helps limit operator-to-operator variability, and is appropriate for laboratories that need consistent preparation of selection media across multiple passages or project teams.

This reagent is supplied in a 10 mL pack size and classified as animal-origin-free, supporting procurement requirements for defined raw-material sourcing. The solution is filter sterilized and intended for aseptic addition to complete culture media at user-qualified working concentrations. Source specifications include liquid form, sterile filtration, 24-month shelf life, frozen light-protected storage, and dry-ice shipment. pH and osmolality are not listed as release specifications for this product; users should qualify final medium parameters after dilution in the relevant formulation. For related antibiotics, buffers, and supplements, see our cell culture reagents category.

• Selection of mammalian cell populations expressing a compatible G418 resistance marker following stable transfection or other gene-introduction workflows.
• Maintenance of selective pressure in established recombinant cell lines during research-scale expansion, banking studies, clone screening, or process-development experiments.
• Support for comparative studies where a sterile, animal-origin-free liquid antibiotic is preferred to reduce preparation time and improve lot-to-lot handling consistency.
• Use in academic, biotechnology, and biopharma laboratories developing recombinant protein expression, reporter-cell systems, genome-engineering readouts, or cell-line characterization assays.
• Preparation of selective complete media after user-defined kill-curve studies. Effective working concentrations vary by cell type, medium composition, cell density, and exposure schedule, so each laboratory should establish selection and maintenance ranges under its own conditions.

Store CCM-RG-0030 at -30 to -5 °C and protect from light. The stated shelf life is 24 months when the container is stored under recommended conditions and handled using aseptic technique. Shipment is on dry ice to maintain frozen temperature control in transit. Upon receipt, verify that the material remains frozen or suitably chilled, transfer promptly to qualified frozen storage, and avoid repeated freeze-thaw cycles by aliquoting when appropriate. Thaw only the amount required for immediate use, mix gently after thawing, and do not use if visible contamination, leakage, or unexpected precipitation is observed. Because G418 activity can vary by cell system, final-use concentrations should be validated internally before routine adoption.

CellCultureMedia supplies CCM-RG-0030 for research, development, and bioprocessing support under ISO-aligned quality practices focused on traceability, controlled handling, and specification review. Each order can be supported with lot-specific documentation, and a Certificate of Analysis is available on request through our quality team. Procurement teams may request current availability, bulk pricing, documentation, and shipping options through the request quote page. The list price for the 10 mL pack is $241.67, with free worldwide shipping available for eligible destinations. For larger programs, CellCultureMedia can coordinate recurring supply, consolidated shipments, and quotation support for multi-site purchasing.