Earle's Balanced Salt Solution, No Phenol Red, Calcium or Magnesium
Earle's Balanced Salt Solution without phenol red, calcium, or magnesium is a liquid balanced salt reagent for maintaining osmotic balance during cell culture workflows. CCM-RG-0014 is supplied as a 500 mL, animal-origin-free, filter-sterilized solution containing D-glucose and sodium bicarbonate. It is configured for laboratories that require a phenol-red-free and divalent-cation-free salt system for washing, dilution, and short-term handling steps. Free worldwide shipping is available, and procurement teams can request a quote for volume supply.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-RG-0014 is an Earle's Balanced Salt Solution (EBSS) formulated for cell culture reagent use where calcium ions, magnesium ions, and phenol red are not required. The formulation includes 1.0 g/L D-glucose as an energy substrate and 2.2 g/L sodium bicarbonate as part of the buffering system. The absence of phenol red supports optical and color-sensitive workflows, while the absence of Ca2+ and Mg2+ is useful in steps where divalent cations may influence cell adhesion, aggregation, or reagent performance.
This product is supplied as a ready-to-use liquid in a 500 mL format. Key release specifications include pH 7.0-7.8, osmolality 260-300 mOsmol/kg, endotoxin ≤1 EU/mL, and filter sterilization. The formulation is classified as animal-origin-free and is positioned within the cell culture reagents portfolio for research, process development, and bioprocess support environments. It is suitable for teams standardizing wash buffers, reagent dilution steps, and non-growth salt solution handling across multiple cell culture platforms.
Applications
- Washing adherent or suspension cell cultures where a balanced inorganic salt matrix is required without added calcium or magnesium.
- Temporary holding, rinsing, or dilution steps during routine cell culture manipulation, media exchange preparation, or sample processing.
- Preparation of cell culture workflows in which phenol red could interfere with absorbance, fluorescence, imaging, or colorimetric readouts.
- Support for dissociation-adjacent handling steps where divalent cation removal is part of the workflow design.
- Use as a standardized EBSS reagent in R&D, QC-support laboratories, pilot operations, and academic core facilities requiring documented pH, osmolality, sterility, and endotoxin attributes.
Storage and Handling
Store CCM-RG-0014 at 2-30 °C protected from light. The product ships at ambient temperature and has a stated shelf life of 48 months when stored unopened under the recommended conditions. Before use, inspect the container for seal integrity, clarity, particulate matter, and expiration dating. Use aseptic technique after opening and minimize repeated warming, prolonged light exposure, and unnecessary container entries. Because the solution contains sodium bicarbonate, maintain appropriate closure practices to limit gas exchange that may affect pH over extended handling periods.
Quality and Documentation
CellCultureMedia supplies CCM-RG-0014 under ISO-aligned manufacturing and quality control expectations for research and bioprocess reagent procurement. Lot-specific documentation can be requested through the quality documentation channel, including Certificate of Analysis availability for qualified orders. The release profile includes filter sterility, endotoxin limit, pH range, osmolality range, animal-origin-free classification, formulation exclusions, and shelf-life information to support vendor qualification and incoming material review. Free worldwide shipping is available for standard orders, and supply planning support is offered for recurring demand, multi-site procurement, and bulk purchasing programs.
Specifications
Formulation Highlights
Contains (+)
1.0 g/L D-glucose, 2.2 g/L sodium bicarbonate
Does Not Contain (-)
Phenol red, Ca 2+, Mg 2+
Storage & Handling
Storage: 2~30 ℃, protected from light. Shelf life: 48 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
About this product
What cell lines does this product support?
How is this product shipped?
Is a Certificate of Analysis available?
What is the typical lead time?
Do you offer bulk pricing?
Learn more about this
- PBS vs DPBS: What Is Different, and Which One to UsePBS and DPBS are both phosphate-buffered saline solutions; the difference is the recipe, not the function. Dulbecco's formulation (DPBS) adds potassium chloride and carries roughly twice the phosphate of a typical PBS (about 9.5 mM versus about 4 mM), and it is sold in two versions - with calcium and magnesium, and without. In practice the with-or-without-divalent-cations choice matters far more than the PBS-or-DPBS label: use a calcium- and magnesium-free solution to wash cells before trypsinisation or EDTA dissociation, because Ca2+ and Mg2+ support the cadherin and integrin bonds you are about to break, and use the version containing calcium and magnesium when cells must stay attached and intact through the wash.
- Cell culture buffersCell culture buffers hold medium in the pH 7.2-7.4 range that mammalian cells require, against the acid load cells generate as they metabolise. The default system in almost every classical medium is sodium bicarbonate working with the CO2 in the incubator atmosphere, which is why bicarbonate content must be matched to the incubator setting: about 1.5-2.2 g/L NaHCO3 for 5% CO2 and 3.7 g/L for 10% CO2. Organic buffers such as HEPES (pKa 7.48) are added at 10-25 mM to hold pH when cultures are outside a CO2 atmosphere, while phosphate-buffered solutions such as PBS and DPBS are used for washing and short-term handling rather than for growth.
- EMEM vs DMEM: What the Modification Actually ChangedEMEM (Eagle's Minimum Essential Medium, also sold as MEM) and DMEM (Dulbecco's Modified Eagle Medium) are the same medium one generation apart: DMEM is Eagle's formulation enriched, with roughly four times the vitamins, about twice most amino acids, twice the glutamine, added glycine and serine, ferric nitrate, and 3.7 g/L sodium bicarbonate against EMEM's 1.5-2.2 g/L. The practical consequence is that DMEM supports fast-growing, metabolically demanding lines such as HEK293 and NIH/3T3, while EMEM suits slower, less demanding adherent cells and primary lines - and because DMEM's higher bicarbonate is formulated for 10% CO2 while EMEM's suits 5%, the two are not interchangeable without checking your incubator.
