Earle's Balanced Salt Solution, No Phenol Red, Calcium or Magnesium

CCM-RG-0014 is an Earle's Balanced Salt Solution (EBSS) formulated for cell culture reagent use where calcium ions, magnesium ions, and phenol red are not required. The formulation includes 1.0 g/L D-glucose as an energy substrate and 2.2 g/L sodium bicarbonate as part of the buffering system. The absence of phenol red supports optical and color-sensitive workflows, while the absence of Ca²⁺ and Mg²⁺ is useful in steps where divalent cations may influence cell adhesion, aggregation, or reagent performance.

This product is supplied as a ready-to-use liquid in a 500 mL format. Key release specifications include pH 7.0-7.8, osmolality 260-300 mOsmol/kg, endotoxin ≤1 EU/mL, and filter sterilization. The formulation is classified as animal-origin-free and is positioned within the cell culture reagents portfolio for research, process development, and bioprocess support environments. It is suitable for teams standardizing wash buffers, reagent dilution steps, and non-growth salt solution handling across multiple cell culture platforms.

• Washing adherent or suspension cell cultures where a balanced inorganic salt matrix is required without added calcium or magnesium.
• Temporary holding, rinsing, or dilution steps during routine cell culture manipulation, media exchange preparation, or sample processing.
• Preparation of cell culture workflows in which phenol red could interfere with absorbance, fluorescence, imaging, or colorimetric readouts.
• Support for dissociation-adjacent handling steps where divalent cation removal is part of the workflow design.
• Use as a standardized EBSS reagent in R&D, QC-support laboratories, pilot operations, and academic core facilities requiring documented pH, osmolality, sterility, and endotoxin attributes.

Store CCM-RG-0014 at 2-30 °C protected from light. The product ships at ambient temperature and has a stated shelf life of 48 months when stored unopened under the recommended conditions. Before use, inspect the container for seal integrity, clarity, particulate matter, and expiration dating. Use aseptic technique after opening and minimize repeated warming, prolonged light exposure, and unnecessary container entries. Because the solution contains sodium bicarbonate, maintain appropriate closure practices to limit gas exchange that may affect pH over extended handling periods.

CellCultureMedia supplies CCM-RG-0014 under ISO-aligned manufacturing and quality control expectations for research and bioprocess reagent procurement. Lot-specific documentation can be requested through the quality documentation channel, including Certificate of Analysis availability for qualified orders. The release profile includes filter sterility, endotoxin limit, pH range, osmolality range, animal-origin-free classification, formulation exclusions, and shelf-life information to support vendor qualification and incoming material review. Free worldwide shipping is available for standard orders, and supply planning support is offered for recurring demand, multi-site procurement, and bulk purchasing programs.