DNase/RNase-Free Distilled Water, 500 mL
DNase/RNase-free distilled water is animal origin-free purified water supplied for cell culture reagent preparation, molecular workflows, and bioprocess support tasks where nuclease control is required. CCM-RG-0020 is packaged as a 500 mL bottle and specified with pH 5.0–7.0, osmolality 0–5 mOsmol/kg, and endotoxin not more than 5 EU/mL. The product ships at room temperature and is offered at $62.50 with free worldwide shipping for qualified B2B orders.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-RG-0020 is DNase/RNase-free distilled water intended for laboratories that require a controlled water source for preparation, dilution, rinsing, and reconstitution steps in cell culture and molecular biology support workflows. It is classified as animal origin-free, making it suitable for procurement programs that restrict animal-derived raw materials in research and process development environments. Supplied in a 500 mL format, it is convenient for shared laboratory benches, media preparation areas, and small-batch reagent production.
The specification profile supports routine use in controlled cell culture operations: pH 5.0–7.0, osmolality 0–5 mOsmol/kg, endotoxin not more than 5 EU/mL, and a 12-month shelf life when stored as directed. These parameters are relevant when water is used in contact with sensitive reagents, serum-free supplements, buffers, and cultureware. For related products used in upstream workflows, see our cell culture reagents category or contact CellCultureMedia for sourcing support.
Applications
- Preparation of cell culture reagent dilutions where DNase/RNase-free water is required by the protocol.
- Reconstitution of powdered or concentrated laboratory reagents used in research cell culture workflows.
- Buffer and rinse solution preparation for non-final formulation process development tasks.
- Support for molecular biology steps adjacent to cell culture, including RNA- or DNA-sensitive sample handling.
- General laboratory water supply for low-osmolality, animal origin-free reagent preparation.
- Small-batch process engineering studies where a documented water input is preferred over unqualified in-house water.
Storage and Handling
Store CCM-RG-0020 at 2–30 °C protected from light. Keep the container tightly closed when not in use and handle with clean laboratory technique to maintain nuclease-free performance after opening. The product ships at room temperature, which simplifies receiving and inventory routing for multi-site procurement teams. Do not use beyond the assigned 12-month shelf life or if the container integrity is compromised. As with all shared laboratory reagents, assign the bottle to a defined work area when possible to reduce cross-use handling risk.
Quality and Documentation
CellCultureMedia supplies CCM-RG-0020 for research, cell culture, and bioprocessing support applications under ISO-aligned supplier quality practices. Lot-specific documentation can confirm applicable release parameters such as pH, osmolality, endotoxin specification, and nuclease-free status. Sterility-related information should be verified on the lot documentation when required for a specific workflow. A Certificate of Analysis is available on request through our quality documentation team. For volume requirements, standing orders, or institutional purchasing, submit a request through request a quote. Free worldwide shipping is available for qualified orders, helping procurement teams consolidate landed cost and reduce freight variability.
Specifications
Storage & Handling
Storage: 2~30 ℃, protected from light. Shelf life: 12 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
About this product
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Learn more about this
- Endotoxin Testing in Cell CultureEndotoxin testing measures bacterial lipopolysaccharide (LPS) in laboratory water, media, sera and reagents, reported in endotoxin units per millilitre (EU/mL), where 1 EU corresponds to roughly 0.1–0.2 ng of reference-standard E. coli endotoxin. The compendial methods are the LAL (limulus amebocyte lysate) assay in its gel-clot, turbidimetric and chromogenic forms under USP <85>, and the animal-free recombinant Factor C (rFC) assay under USP <86>; kinetic formats quantify down to about 0.001–0.005 EU/mL. Practical cell-culture limits are 0.25 EU/mL for water, ≤1 EU/mL for most cell-culture-grade reagents, and ≤10 EU/mL as the general industry standard for fetal bovine serum, with low-endotoxin grades at ≤1 EU/mL and ultra-low grades below 0.1 EU/mL. Endotoxin survives autoclaving and passes 0.2 µm filters, so it must be excluded at source rather than removed later.
- Poly-L-Lysine Coating for Cell CulturePoly-L-lysine coating covers a culture surface with a positively charged synthetic polymer that binds the negatively charged plasma membrane, allowing weakly adherent cells to attach to glass or plastic. The standard protocol applies a sterile 0.01% (0.1 mg/mL) solution at roughly 1 mL per 25 cm2, leaves it in contact with the surface for 5 minutes to 1 hour at room temperature, aspirates, rinses thoroughly with sterile tissue-culture-grade water, and air-dries the surface for at least 2 hours before cells are seeded. The attachment it provides is purely electrostatic: poly-L-lysine does not engage integrins, so cells that need genuine extracellular matrix signalling require laminin, fibronectin or collagen instead of it or on top of it.
