Collagenase Type I Solution, 1 mg/mL

CCM-RG-0056 is a ready-to-use collagenase Type I solution supplied at 1 mg/mL for laboratories that require consistent enzyme input without in-house powder weighing or reconstitution. Collagenase Type I is commonly selected for digestion of collagen-rich matrices and release of adherent or embedded cells during sample preparation, primary cell isolation research, 3D culture processing, and upstream method development. The animal origin-free classification supports risk-managed sourcing programs and simplifies procurement review for organizations that restrict animal-derived components in cell culture reagents.

The product is packaged as 10 mL and priced from $97.22 per unit. Key release specifications include endotoxin ≤10 EU/mL and a 24-month shelf life when stored as directed. pH and osmolality are not specified for this enzyme reagent; users should qualify performance in the complete buffer, medium, and cell handling workflow. For adjacent reagents, media, and supplements, see the cell culture reagents category.

• Enzymatic dissociation of collagen-containing extracellular matrix in cell culture preparation workflows.
• Research-scale processing of adherent cultures, tissue-derived samples, spheroids, matrices, and scaffold-associated cell models where collagen digestion is required.
• Process development studies comparing enzyme concentration, incubation time, agitation, temperature, and quench conditions.
• Preparation steps for cell viability, recovery, and yield assessments in R&D and scale-down bioprocess models.
• Procurement programs requiring an animal origin-free classification, controlled shelf life, and lot-specific documentation for supplier qualification.

Store CCM-RG-0056 at -30 to -5 ℃ and protect from light. The product ships on dry ice to maintain frozen-condition integrity during transit. Upon receipt, verify that the container remains frozen or appropriately chilled, record lot details, and transfer promptly to qualified frozen storage. Avoid repeated freeze-thaw cycles where possible; aliquot under appropriate aseptic laboratory practice if repeated access is expected. Thaw only the volume required for the planned procedure and keep exposure time at working temperature consistent across validated runs. Do not use beyond the stated 24-month shelf life or after internal acceptance criteria are exceeded.

CellCultureMedia supplies CCM-RG-0056 for B2B research and bioprocessing customers that need clear specifications, controlled logistics, and responsive documentation support. Manufacturing and supplier controls are managed within ISO-aligned quality expectations, and a Certificate of Analysis is available on request for lot review. The CoA can support incoming inspection, endotoxin confirmation, shelf-life verification, and purchasing record retention. For documentation expectations and supplier qualification support, visit quality and documentation. For larger quantities, scheduled supply, or multi-site purchasing, submit requirements through request a quote; eligible orders include free worldwide shipping with dry ice logistics where required.