Antibiotic-Antimycotic Triple Solution 100X

CCM-RG-0027 is a 100X antibiotic-antimycotic triple solution formulated for addition to cell culture media where bacterial and fungal bioburden control is required during in vitro culture operations. The reagent combines 10,000 units penicillin per mL, 10,000 μg streptomycin per mL, and 25 μg amphotericin B per mL in a ready-to-use liquid concentrate. At a typical 1X working concentration, the formulation delivers broad coverage for routine media supplementation while allowing laboratories to standardize a single additive across multiple compatible media systems.

The solution is manufactured as an animal-origin-free cell culture reagent and is sterile filtered before release. Key release specifications include pH 5.2–7.0 and osmolality 305–350 mOsmol/kg, supporting predictable dilution into aqueous media without introducing excessive osmotic variation at standard use levels. The product is assigned to the cell culture antibiotics category and is intended for research, development, and bioprocessing use only. For adjacent media additives and related buffers, see the cell culture reagents category.

• Routine adherent and suspension cell culture: Add to compatible complete media to help manage bacterial and fungal contamination pressure during cell expansion, maintenance, and experimental setup.
• Primary culture and low-passage workflows: Useful where sample handling or extended manipulation increases the need for a defined antibiotic-antimycotic supplement, subject to lab-specific validation.
• Process development studies: Suitable for upstream development teams assessing media handling robustness, culture stability, and reagent compatibility in shake flasks, spinner systems, multiwell plates, and small-scale bioreactors.
• Media preparation operations: The 100X concentration reduces storage footprint and supports efficient preparation of larger media batches by adding 10 mL per liter for a 1X final concentration, unless an internal protocol specifies otherwise.
• Academic and industrial R&D: Provides a defined three-component antimicrobial additive for laboratories that require consistent lot documentation, controlled storage conditions, and procurement-ready specifications.

Store CCM-RG-0027 at -30 to -5 °C protected from light. The specified shelf life is 24 months when the product remains within the stated storage range and is handled using aseptic technique. Shipments are packed on dry ice; upon receipt, verify that the container remains frozen or cold, record receipt condition according to site procedures, and transfer promptly to qualified frozen storage. Avoid repeated freeze-thaw cycling by aliquoting under sterile conditions where repeated access is expected. Thaw only the volume required for preparation, mix gently to ensure homogeneity, and do not use if the solution shows unexpected turbidity, particulate matter, damaged packaging, or evidence of compromised closure integrity. Because the reagent is supplied as a concentrated additive, users should confirm compatibility with the target medium, cell type, assay readout, and downstream analytics before implementation in controlled workflows.

CellCultureMedia supplies CCM-RG-0027 for organizations that require specification-grade reagents, traceable documentation, and practical procurement support. The product is produced in an ISO-aligned manufacturing environment with sterile filtration as the stated sterility method, animal-origin-free classification, defined pH and osmolality ranges, and controlled frozen distribution. Lot-specific documentation, including a Certificate of Analysis, can be requested through the quality documentation team for incoming QC review, supplier qualification files, or internal batch records.

The 100 mL pack size is suitable for laboratories preparing approximately 10 L of 1X supplemented medium at the common 1:100 dilution, subject to validated site procedures. Current listed price is $161.11 per bottle, with free worldwide shipping available and dry-ice logistics used to preserve frozen condition in transit. For standing orders, multi-bottle purchases, distributor supply, or project-based demand planning, submit requirements through request a quote so the team can confirm availability, lead time, and commercial terms.