NK Cell Serum-Free Medium, Phenol Red-Free

CCM-SF-0068 is designed for procurement teams, process engineers, and R&D groups that require a defined serum-free medium for NK cell expansion and maintenance studies. The product is supplied as a ready-to-use liquid and does not require serum supplementation, helping reduce variability associated with serum-containing systems. Its animal-origin-free classification supports laboratories standardizing raw-material inputs for cell culture development, scale-up evaluation, and controlled process comparison.

The medium is phenol red-free, which is useful when colorimetric interference must be minimized in optical readouts, metabolite monitoring, or automated imaging workflows. Key formulation components include D-glucose for carbon source support, L-glutamine and alanyl-glutamine for nitrogen metabolism and enhanced glutamine stability, and sodium bicarbonate for CO2-buffered culture. Source specifications define a pH range of 7.0–7.4 and osmolality of 280–320 mOsmol/kg, aligning with common mammalian suspension and immune-cell culture environments. The recommended culture setting is 37°C with 4–6% CO2.

• Serum-free culture of NK cells in research, process-development, and cell-processing model workflows.
• Animal-origin-free media qualification studies where lot consistency, documentation, and defined inputs are required.
• Phenol red-free culture conditions for imaging, absorbance-based assays, fluorescence workflows, or media color-interference reduction.
• Small-scale shake flask, static culture, and vessel-transition studies prior to larger process formats, subject to in-house verification.
• Comparative medium screening for NK cell viability, density, metabolite profile, and expansion performance under 37°C and 4–6% CO2 incubation.
• Procurement programs requiring a 1 L liquid format with defined sterility, endotoxin, pH, osmolality, storage, and shelf-life specifications.

Store CCM-SF-0068 at 2–8°C protected from light. The product is shipped on blue ice to maintain refrigerated conditions during transit; do not freeze unless an internally validated protocol supports that practice. Handle aseptically after opening and minimize repeated warming cycles by aliquoting into sterile containers when appropriate for the laboratory workflow. The specified shelf life is 12 months when stored under recommended conditions. Before use, equilibrate only the required volume to working temperature and inspect the liquid for container integrity, clarity, and visible particulates according to local quality procedures.

CCM-SF-0068 is filter-sterilized and specified with endotoxin at ≤1 EU/mL. Manufacturing and release practices are managed under ISO-aligned quality expectations, with documentation intended to support supplier qualification, incoming material review, and controlled research or bioprocessing use. A Certificate of Analysis is available on request through CellCultureMedia quality support, and procurement teams can request current lot details, bulk availability, and shipping documentation through the request quote workflow. Free worldwide shipping is available where applicable, with temperature-controlled dispatch on blue ice.