Xeno-Free Serum-Free Lymphocyte Medium with HSA

CCM-SC-0011 is designed for lymphocyte culture where procurement teams and process scientists require defined composition, animal-component control, and lot documentation. The medium is serum-free and classified as xeno-free, with human serum albumin included to support protein supplementation while avoiding animal serum. The absence of phenol red is useful for fluorescence-based assays, optical monitoring, and workflows where indicator dye can interfere with analytical readouts.

The product is supplied as a liquid medium for incubation at 37°C in 5–10% CO2. Key release specifications include pH 7.0–7.4, osmolality 280–330 mOsmol/kg, endotoxin not more than 3 EU/mL, and sterile-filtered final product status. The dual glutamine system combines 1.5 mM L-glutamine with 1.25 mM L-alanyl-glutamine to provide immediate and more stable glutamine availability during culture. For related defined media, see the stem cell media category or use request a quote for volume planning.

• Lymphocyte culture in research, assay development, and controlled process-development studies.
• Phenol red-free workflows where absorbance, fluorescence, imaging, or metabolite monitoring may be affected by color indicators.
• Serum-free and xeno-free protocols requiring reduced undefined serum contribution and more consistent lot-to-lot input control.
• Expansion and maintenance studies for lymphocyte populations, including T-cell, B-cell, or NK-cell workflows when paired with the user’s validated cytokines, activation reagents, matrices, and process parameters.
• Cell and gene processing research where documented medium attributes, endotoxin control, and defined storage conditions are important for scale-up planning.
• Comparability studies between medium lots, supplier qualification programs, and academic or biopharma R&D programs that require clear formulation attributes and CoA traceability.

Store CCM-SC-0011 at 2–8°C protected from light. Do not freeze unless a validated internal procedure supports freezing of this medium format. The stated shelf life is 12 months when stored unopened under recommended conditions. Shipments are packed on blue ice to maintain refrigerated transit conditions, and receiving teams should transfer bottles to 2–8°C storage promptly upon arrival. Use aseptic technique after opening, minimize repeated warming cycles, and record lot number, opening date, and in-use hold time according to site procedures.

CellCultureMedia supplies CCM-SC-0011 for research and bioprocessing use with specification-driven documentation to support purchasing, incoming inspection, and technical evaluation. Manufacturing and quality controls are ISO-aligned, and a Certificate of Analysis is available on request with lot-specific results for key release criteria such as pH, osmolality, sterility status, and endotoxin. Additional quality information can be reviewed through our quality documentation resource. Free worldwide shipping is available, and bulk pricing can be quoted for standing orders, multi-lot planning, or pilot-scale evaluation.