Xeno-Free Serum-Free Lymphocyte Medium

CCM-SC-0012 is a ready-to-use serum-free medium designed for lymphocyte expansion and maintenance where animal-origin serum is not desired. The xeno-free liquid formulation supports controlled research and process-development workflows that require defined inputs, reduced serum variability, and straightforward scale planning. It is supplied with phenol red for visual pH indication, D-glucose as the primary carbohydrate source, both L-glutamine and L-alanyl-L-glutamine for nitrogen support, and sodium bicarbonate for CO2-buffered incubation.

The product is intended for 37 °C incubation with 5–10% CO2 and is classified as a serum-free medium for lymphocyte culture. Release specifications include pH 7.0–7.4, osmolality 280–330 mOsmol/kg, endotoxin ≤1 EU/mL, and sterile-filtered processing. For related defined media options, see the stem cell media category. Procurement teams can reference CCM-SC-0012 for the 1 L bottle configuration and a listed unit price of $483.33 before any contract, standing-order, or bulk adjustments.

• Lymphocyte culture in serum-free, xeno-free research and process-development environments.
• Feasibility studies comparing serum-free media performance against serum-containing internal controls.
• Expansion workflow optimization where controlled pH, osmolality, and CO2 buffering are documented requirements.
• Closed or semi-closed culture process planning that requires sterile-filtered liquid medium with defined storage and shipping conditions.
• R&D screening of activation, feeding, seeding density, and passage parameters for lymphocyte platforms.
• Procurement standardization for laboratories seeking a 1 L liquid format with documented endotoxin, pH, osmolality, and shelf-life specifications.

Store CCM-SC-0012 at 2–8 °C protected from light. Do not freeze unless validated internally, as freeze-thaw exposure may alter performance of liquid media components. The product ships on blue ice and should be transferred to refrigerated storage promptly upon receipt. Use aseptic handling for all dispensing steps, minimize open-container time, and pre-warm only the volume required for immediate use. The assigned shelf life is 12 months when stored unopened under the recommended refrigerated, light-protected conditions. Before use, confirm that the medium is clear, within expiry, and appropriate for the intended cell culture protocol.

CellCultureMedia supplies CCM-SC-0012 for research, development, and bioprocessing use with specification-driven documentation for purchasing and technical review. Manufacturing and release controls are ISO-aligned, and batch documentation can be requested to support incoming material qualification. A Certificate of Analysis is available on request, including key release attributes such as sterility status, endotoxin limit, pH range, osmolality range, shelf life, and storage conditions. Review our documentation approach at quality and compliance, or use request a quote for bulk pricing, standing orders, and destination-specific logistics. Free worldwide shipping is available for eligible orders, with blue-ice packing used for this refrigerated liquid medium.