Animal-Origin-Free ITS-X 100X Supplement
Animal-origin-free ITS-X 100X supplement is a sterile liquid cell culture additive containing recombinant human insulin, recombinant human transferrin, sodium selenite, and ethanolamine for serum-reduced or serum-free workflows. CCM-RG-0074 is supplied in a 10 mL format for R&D, process development, and routine culture support. The formulation is available at USD 333.33 with free worldwide shipping, subject to destination handling requirements. Use it when a defined, non-animal-origin supplement is required for reproducible cell culture media preparation.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-RG-0074 is a 100X animal-origin-free ITS-X cell culture supplement for defined media systems. The liquid formulation contains 1.0 g/L recombinant human insulin, 550 mg/L recombinant human transferrin, 0.67 mg/L sodium selenite, and 200 mg/L ethanolamine. It is intended for 1:100 addition unless the user’s validated protocol specifies another concentration. The product is sterile filtered and supplied as a ready-to-use stock solution, reducing weighing, dissolution, and filtration steps in media preparation.
This supplement is designed for laboratories and bioprocess teams that need consistent micronutrient and carrier-protein support while limiting animal-derived inputs. Insulin supports uptake and metabolism of key nutrients; transferrin provides an iron-binding component; selenium is supplied as sodium selenite; and ethanolamine supports membrane lipid synthesis. The specification range is pH 7.0-9.0 and osmolality 260-320 mOsmol/kg. For related defined additives, see our cell culture reagents category.
Applications
- Supplementation of serum-reduced and serum-free mammalian cell culture media in research and process development settings.
- Defined media optimization for adherent or suspension cell systems where insulin, transferrin, selenium, and ethanolamine are required in a single stock.
- Support for clone screening, media comparison studies, scale-down process models, and routine culture maintenance.
- Preparation of small-batch media where a 10 mL 100X format is preferred for controlled consumption and reduced waste.
- Procurement programs requiring animal-origin-free classification, sterile filtration, endotoxin control, and lot-specific documentation.
Storage and Handling
Store CCM-RG-0074 at 2-8 °C protected from light. The product is shipped on blue ice to maintain refrigerated conditions during transit. Do not leave the bottle at room temperature for extended periods, and minimize repeated warming events during use. Handle aseptically in a suitable biosafety cabinet or clean work area after opening. The stated shelf life is 24 months when stored unopened under recommended conditions. Inspect the container and solution before use; do not use material that shows visible contamination, unexpected turbidity, or compromised closure integrity.
Quality and Documentation
CCM-RG-0074 is produced under ISO-aligned manufacturing and quality-control practices with lot traceability for procurement and audit support. Release specifications include sterile filtration, endotoxin not more than 1 EU/mL, pH 7.0-9.0, and osmolality 260-320 mOsmol/kg. A lot-specific Certificate of Analysis is available on request through our quality documentation team. The 10 mL unit is listed at USD 333.33 and includes free worldwide shipping where import and cold-chain lanes are available. For standing orders, consolidated purchasing, or volume requirements, submit details via request a quote.
Specifications
Formulation Highlights
Contains (+)
1.0 g/L recombinant human insulin, 550 mg/L recombinant human transferrin, 0.67 mg/L sodium selenite, 200 mg/L ethanolamine
Storage & Handling
Storage: 2~8 ℃, protected from light. Shelf life: 24 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
About this product
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Learn more about this
- Recombinant Human Albumin in Cell CultureRecombinant human serum albumin (rHSA) is a 585-amino-acid, 66.5 kDa non-glycosylated protein produced in yeast such as *Pichia pastoris* or in transgenic rice rather than purified from human plasma, and it is used as an animal-origin-free supplement in serum-free and chemically defined culture media. In culture it works as a carrier for fatty acids, lipids, hormones and trace elements, as an antioxidant through its free cysteine-34 thiol, as a scavenger that sequesters toxic metals and excess free fatty acids, and as a shear-protective surface-active protein in stirred and sparged culture. Typical working concentrations are 0.1-10 g/L, most often 0.5-5 g/L, and it is commonly supplied as a 200 mg/mL (20%) solution. Its performance depends heavily on what is bound to it, so fatty-acid-loaded and fatty-acid-free preparations behave quite differently.
- Recombinant Human Insulin in Cell CultureRecombinant human insulin is a 51-amino-acid, 5.8 kDa two-chain protein used as a core supplement in serum-free and chemically defined culture media, where it drives glucose and amino acid uptake, protein and lipid synthesis, and cell survival. It is produced in *E. coli* or yeast rather than extracted from animal pancreas, making it animal-origin-free, and is usually supplied as a zinc-stabilised dry powder. The typical working concentration is 5-10 ug/mL, the level delivered by a standard 1X ITS supplement, which is roughly a thousandfold above physiological insulin levels -- at that concentration insulin acts substantially through the IGF-1 receptor as well as its own. Because insulin is poorly soluble near neutral pH, powder must be dissolved in dilute hydrochloric or acetic acid before dilution into medium.
