Animal-Origin-Free ITS-X 100X Supplement

CCM-RG-0074 is a 100X animal-origin-free ITS-X cell culture supplement for defined media systems. The liquid formulation contains 1.0 g/L recombinant human insulin, 550 mg/L recombinant human transferrin, 0.67 mg/L sodium selenite, and 200 mg/L ethanolamine. It is intended for 1:100 addition unless the user’s validated protocol specifies another concentration. The product is sterile filtered and supplied as a ready-to-use stock solution, reducing weighing, dissolution, and filtration steps in media preparation.

This supplement is designed for laboratories and bioprocess teams that need consistent micronutrient and carrier-protein support while limiting animal-derived inputs. Insulin supports uptake and metabolism of key nutrients; transferrin provides an iron-binding component; selenium is supplied as sodium selenite; and ethanolamine supports membrane lipid synthesis. The specification range is pH 7.0-9.0 and osmolality 260-320 mOsmol/kg. For related defined additives, see our cell culture reagents category.

• Supplementation of serum-reduced and serum-free mammalian cell culture media in research and process development settings.
• Defined media optimization for adherent or suspension cell systems where insulin, transferrin, selenium, and ethanolamine are required in a single stock.
• Support for clone screening, media comparison studies, scale-down process models, and routine culture maintenance.
• Preparation of small-batch media where a 10 mL 100X format is preferred for controlled consumption and reduced waste.
• Procurement programs requiring animal-origin-free classification, sterile filtration, endotoxin control, and lot-specific documentation.

Store CCM-RG-0074 at 2-8 °C protected from light. The product is shipped on blue ice to maintain refrigerated conditions during transit. Do not leave the bottle at room temperature for extended periods, and minimize repeated warming events during use. Handle aseptically in a suitable biosafety cabinet or clean work area after opening. The stated shelf life is 24 months when stored unopened under recommended conditions. Inspect the container and solution before use; do not use material that shows visible contamination, unexpected turbidity, or compromised closure integrity.

CCM-RG-0074 is produced under ISO-aligned manufacturing and quality-control practices with lot traceability for procurement and audit support. Release specifications include sterile filtration, endotoxin not more than 1 EU/mL, pH 7.0-9.0, and osmolality 260-320 mOsmol/kg. A lot-specific Certificate of Analysis is available on request through our quality documentation team. The 10 mL unit is listed at USD 333.33 and includes free worldwide shipping where import and cold-chain lanes are available. For standing orders, consolidated purchasing, or volume requirements, submit details via request a quote.