Animal-Origin-Free ITS-G 100X Supplement

This ITS-G supplement is a concentrated liquid additive for use in mammalian cell culture media where defined, animal-origin-free components are required. The 100X format is intended for 1:100 dilution into compatible base media, allowing procurement teams and laboratory managers to standardize small-volume supplementation without preparing individual stock solutions. Each liter of concentrate contains 1.0 g recombinant human insulin, 550 mg recombinant human transferrin, and 0.67 mg sodium selenite. At the typical 1X working concentration, these components contribute 10 mg/L insulin, 5.5 mg/L transferrin, and 0.0067 mg/L sodium selenite.

The formulation is classified as animal-origin-free and is supplied in liquid form for convenient aseptic addition. Insulin is commonly used to support glucose uptake and metabolism in cultured cells, transferrin provides a defined iron-binding component, and selenium is supplied as sodium selenite for trace-element supplementation. CCM-RG-0072 is part of our cell culture reagents portfolio for laboratories that need documented, specification-driven inputs for research and bioprocess development.

• Defined supplementation of serum-free, serum-reduced, or low-protein mammalian cell culture media during R&D and process-development studies.
• Evaluation of insulin, transferrin, and selenium requirements in adherent or suspension culture systems where animal-origin-free inputs are preferred.
• Media optimization experiments requiring a consistent 100X ITS-G source rather than separately weighed or mixed component stocks.
• Support for routine maintenance, expansion, and assay preparation workflows in academic, biotech, and biopharma laboratories.
• Use as a cell culture-related reagent in controlled procurement programs where sterility, endotoxin limit, shelf life, and storage conditions are required on purchasing documentation.

Store CCM-RG-0072 at 2–8 °C protected from light. The product is shipped on blue ice to maintain refrigerated conditions during transit; do not freeze unless your internal validation supports freeze-thaw handling for the intended workflow. Use aseptic technique when opening the 10 mL container and minimize repeated warming or extended room-temperature exposure. The stated shelf life is 24 months when stored unopened under the recommended conditions. After opening, laboratories should assign an internal in-use period based on sterility controls, container handling, and local quality procedures.

CCM-RG-0072 is sterile-filtered and specified with an endotoxin limit of ≤1 EU/mL. Manufacturing and release controls are aligned with ISO-style quality practices for research and bioprocessing reagents, including lot traceability and documented specification review. A Certificate of Analysis can be requested through our quality documentation team, and procurement quotes are available through the request quote workflow for standing orders, bulk packs, or multi-site supply planning. Free worldwide shipping is available, with cold-chain packaging selected according to destination and shipment duration.