Animal-Origin-Free Cell Freezing Medium, High Density
Animal-origin-free cell freezing medium, high density, is a ready-to-use liquid reagent for cryopreservation workflows in cell culture, research, and bioprocessing. CCM-RG-0092 is supplied as a 100 mL bottle for laboratories that require a defined freezing solution without animal-derived components. The formulation is sterile filtered, liquid, and specified for pH 7.0–7.4 with osmolality of 1000–1500 mOsm/kg H2O. CellCultureMedia offers this item at USD 805.56 with free worldwide shipping and quotation support for recurring purchasing.
- ✓ Free worldwide shipping
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
- ✓ Bulk & custom volumes quoted on request
Overview
CCM-RG-0092 is an animal-origin-free cell freezing medium designed for cell preservation liquid workflows, frozen cell banking, and process-development studies where defined reagent sourcing is required. The product is supplied as a liquid in a 100 mL format and is intended for use as part of controlled freezing and post-thaw recovery protocols established by the user. Its high-osmolality specification supports the concentrated solute environment typically required in freezing media, while the animal-origin-free classification helps procurement teams standardize material inputs for documented cell-culture operations.
Key release specifications include sterile filtration, pH 7.0–7.4, osmolality 1000–1500 mOsm/kg H2O, and a 24-month shelf life when stored as directed. The product category is cell preservation and freezing media within cell culture reagents. It is suitable for R&D laboratories, cell repository teams, and bioprocess groups that need a defined, ready-to-use freezing reagent rather than an internally compounded solution.
Applications
- Preparation of frozen working cell banks and research cell stocks using user-validated controlled-rate freezing protocols.
- Animal-origin-free cell culture workflows where serum-containing freezing mixtures are being reduced or replaced for material-control reasons.
- High-density cell suspension freezing studies during upstream process development, media screening, or cell line evaluation.
- Academic, biotech, and biopharma laboratory workflows requiring a ready-to-use liquid reagent with defined pH, osmolality, sterility, and shelf-life specifications.
- Standardization of freezing reagent inputs across multiple sites, instruments, or project teams. For planned demand, buyers may request a quote for standing orders, multi-bottle purchases, or scheduled replenishment.
Storage and Handling
Store CCM-RG-0092 at 2–30 °C protected from light. The product ships at ambient temperature, which simplifies receiving for international procurement and multi-site distribution. Keep the bottle tightly closed when not in use and handle aseptically in a suitable clean workspace to maintain sterility after opening. Before use, mix gently without foaming and verify that the liquid is clear and within the laboratory’s acceptance criteria. Do not use beyond the assigned shelf life or after exposure to storage conditions outside the stated range unless an internal quality assessment supports continued use.
Quality and Documentation
CellCultureMedia supplies CCM-RG-0092 through ISO-aligned manufacturing and quality practices appropriate for research and bioprocess reagent supply. Lot-specific documentation is available on request and may include Certificate of Analysis details for appearance, sterility by filtration process, pH, osmolality, shelf life, and relevant lot identifiers. Documentation support helps quality, procurement, and process teams maintain traceability during qualification and routine ordering. Visit quality documentation for general information, or contact CellCultureMedia for lot documentation before purchase. Free worldwide shipping is available for this 100 mL format.
Specifications
Storage & Handling
Storage: 2~30 ℃, protected from light. Shelf life: 24 months. Ship cold-chain where required.
Quality & Documentation
Manufactured under ISO-aligned quality management systems. Full documentation — Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), formulation summary, and stability data — is available on request. Email [email protected] with the SKU.
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Learn more about this
- Freezing Medium for Cells and Cryopreservation ProtocolsCell freezing medium is a cryoprotectant-containing solution that allows cells to survive freezing and long-term storage, most commonly built from a base medium or serum plus 5-10% dimethyl sulfoxide (DMSO), with 10% the standard starting point. DMSO permeates the cell and modifies how water freezes, preventing the intracellular ice crystals that otherwise rupture membranes during cooling. Cells are frozen at a controlled rate of approximately 1 C per minute to -80 C and then transferred to the vapour phase of liquid nitrogen, since long-term storage requires temperatures below about -135 C to prevent ice recrystallisation. Thawing is the opposite -- as rapid as possible in a 37 C water bath, followed by prompt dilution to remove the DMSO, which is toxic to cells above freezing temperatures.