Cell Dissociation Medium with Phenol Red and EDTA

This dissociation reagent is formulated as a ready-to-use liquid for releasing adherent cells from culture vessels while supporting controlled handling in laboratory and process-development settings. The formulation includes phenol red as a visual pH indicator and 1 mM EDTA to assist cell detachment by chelating divalent cations involved in cell-surface and substrate interactions. Its animal origin-free classification supports workflows where reagent traceability, defined inputs, and reduced animal-derived component exposure are procurement requirements.

CCM-RG-0050 is supplied sterile-filtered with a specification pH range of 7.0 to 7.4 and osmolality of 270 to 340 mOsmol/kg. Endotoxin is specified at ≤1 EU/mL, supporting use in sensitive culture environments where low bioburden and lot consistency are important. The product sits within our cell culture reagents portfolio and is intended for in vitro cell culture, R&D, assay preparation, passaging, scale-up evaluation, and upstream process support. The 500 mL bottle size is suitable for routine laboratory consumption as well as multi-user core facilities that consolidate reagent purchasing under documented specifications.

• Detachment of adherent mammalian cell cultures during routine passaging, expansion, or harvest preparation.
• R&D comparison of animal origin-free dissociation reagents in cell line development, media optimization, and process characterization studies.
• Preparation of single-cell or low-clump suspensions prior to counting, viability assessment, seeding, cryopreservation, or analytical testing.
• Use in workflows where a phenol red indicator is useful for quick visual confirmation of pH status during handling.
• Support for procurement programs requiring sterile-filtered reagents, defined storage conditions, endotoxin limits, and lot-specific documentation.

Store at 2 to 8 °C and protect from conditions that may compromise sterility or performance. The product is shipped on blue ice and should be transferred to refrigerated storage promptly upon receipt. Do not use if the container is damaged, visibly contaminated, or outside internal acceptance criteria. Use aseptic technique when withdrawing material, minimize repeated warm-up cycles, and record lot number, opening date, and operator according to site procedures. The stated shelf life is 24 months when stored unopened under recommended conditions. For high-throughput or regulated internal workflows, users should qualify the reagent with their own cell types, vessel formats, incubation timing, and downstream acceptance criteria before routine implementation.

CellCultureMedia supplies CCM-RG-0050 for professional cell culture procurement programs that require clear specifications, stable sourcing, and responsive documentation support. Manufacturing and release are managed under ISO-aligned quality principles, with sterility, endotoxin, pH, osmolality, shelf life, storage, and shipping conditions available for review. A Certificate of Analysis can be requested through the quality documentation team, and purchasing teams may use the request a quote workflow to confirm price, lead time, batch availability, and bulk order options. Free worldwide shipping is available where logistics lanes and order conditions qualify.