Animal-Origin-Free Cell Culture Freezing Medium
Animal-origin-free cell culture freezing medium is a sterile, ready-to-use cryostorage reagent formulated with 7.5% DMSO for controlled storage of cultured cells. CCM-RG-0091 is supplied as a 100 mL liquid reagent for research, process development, and bioproduction workflows requiring animal-derived component-free inputs. The formulation is sterile-filtered, specified at pH 7.0-8.0 and 1200-1500 mOsmol/kg, and ships on blue ice with shipping quoted per order for qualified B2B orders.
- ✓ Wholesale and bulk pricing on request
- ✓ Private-label and OEM packaging quoted on request
- ✓ ISO-aligned manufacturing
- ✓ Certificate of Analysis on request
Wholesale supply terms
- Pack size
- Listed pack: 100 mL. Other pack sizes on request.
- Minimum order
- Minimum order quantities on request
- Bulk format
- Bulk packs quoted on request; ask whether a powder format is available
- Private label / OEM
- Private-label and OEM packaging quoted on request
- Lot reservation
- Lot reservation for repeat supply on request
- Lead time
- Quoted per order
- Documents
- Certificate of Analysis on request
We supply companies that resell to laboratories: distributors, resellers, catalogue companies, marketplaces and private-label buyers. We do not sell single units to individual labs.
Overview
CCM-RG-0091 is an animal-origin-free cell culture freezing medium intended for standardized cell banking and frozen cell transfer workflows. The liquid format reduces preparation time versus in-house mixing, while the 7.5% DMSO content supports controlled cooling protocols used for many mammalian cell culture systems. It is positioned within our cell culture reagents portfolio for teams that need consistent procurement specifications, lot documentation, and dependable global fulfillment.
Key specifications include sterile-filtered processing, animal-origin-free classification, pH 7.0-8.0, osmolality 1200-1500 mOsmol/kg, and a 24-month shelf life when stored as directed. It is suitable for labs replacing serum-containing or animal-derived freezing mixtures with a defined, ready-to-use reagent for reproducible cell handling across R&D and process development environments.
Applications
- Creation and maintenance of working cell banks for research cell lines, suspension cultures, and adherent mammalian cultures.
- Freezing of cultured cells during clone screening, assay development, media evaluation, and upstream process optimization.
- Support for animal-derived component-free workflows where procurement teams need defined inputs and clear documentation.
- Controlled storage of cell stocks used in routine passaging, scale-up studies, transfection workflows, and cell-based analytical assays.
- Standardized frozen aliquot preparation for multi-site R&D programs, contract research operations, and academic core facilities.
Storage and Handling
Store at 2-30 °C and protect from light. The product is shipped on blue ice to support temperature-controlled delivery during transit. Before use, equilibrate the container to the intended working temperature according to the receiving laboratory’s validated procedure, mix gently if needed, and use aseptic technique when transferring into freezing vials or culture vessels. Avoid repeated unnecessary exposure to light and keep the container closed when not dispensing. Do not use if the container integrity is compromised, if visible contamination is present, or if the material is past its assigned expiration date.
Quality and Documentation
CellCultureMedia supplies CCM-RG-0091 for research and bioprocessing use with ISO-aligned supplier controls, lot traceability, and documentation support for purchasing and technical review. A Certificate of Analysis can be requested through our quality documentation process, including confirmation of relevant lot specifications such as appearance, sterility status, pH, osmolality, and release date when available. Endotoxin information may be provided when included in the lot documentation.
For planned campaigns, recurring orders, or multi-site supply, procurement teams can use the request a quote channel to confirm current pricing, availability, lead time, and bulk pack options. Shipping is quoted per order and destination; wholesale quantities ship most economically. CellCultureMedia can coordinate shipping documentation and commercial paperwork for institutional buyers, distributors, and bioprocessing groups requiring predictable international supply.
What to send for a quotation
- Product and SKU (CCM-RG-0091)
- Pack size (100 mL)
- Quantity per quarter
- Repeat cadence
- Destination country
Terms, minimums and lead time are stated in the written quotation for that order. Private label and lot reservation can be requested; both are quoted per project or order. See the distributor programme for how we work with resellers.
Specifications
Formulation highlights
Contains (+)
[+]7.5% DMSO
Storage and handling
Storage: 2~30 ℃, protected from light. Shelf life: 24 months. Shipped cold-chain where required.
Quality and documentation
Manufactured under ISO-aligned quality management systems. A Certificate of Analysis is available on request: email info@cellculturemedia.bio with the SKU.
About this product
What cell lines does this product support?
How is this product shipped?
Is a Certificate of Analysis available?
What is the typical lead time?
Do you offer bulk pricing?
Learn more about this
- DF-1 Chicken Embryo Fibroblast CellsDF-1 (UMNSAH/DF-1, ATCC CRL-12203) is a spontaneously immortalised chicken embryo fibroblast line derived from East Lansing Line 0 embryos, a chicken line free of endogenous avian leukosis virus loci. Because it carries no endogenous retrovirus and was immortalised without viral or chemical transformation, it is the standard continuous substitute for primary chicken embryo fibroblasts in avian virology, vaccine development and recombinant virus production. DF-1 cells are cultured in high-glucose DMEM with 10% fetal bovine serum at 37 C in 5% CO2, grow faster than primary CEF, transfect efficiently, and support replication of avian influenza, infectious bursal disease virus, avian leukosis and sarcoma viruses and many other avian pathogens at titres equal to or higher than CEF.
- Freezing Medium for Cells and Cryopreservation ProtocolsCell freezing medium is a cryoprotectant-containing solution that allows cells to survive freezing and long-term storage, most commonly built from a base medium or serum plus 5-10% dimethyl sulfoxide (DMSO), with 10% the standard starting point. DMSO permeates the cell and modifies how water freezes, preventing the intracellular ice crystals that otherwise rupture membranes during cooling. Cells are frozen at a controlled rate of approximately 1 C per minute to -80 C and then transferred to the vapour phase of liquid nitrogen, since long-term storage requires temperatures below about -135 C to prevent ice recrystallisation. Thawing is the opposite -- as rapid as possible in a 37 C water bath, followed by prompt dilution to remove the DMSO, which is toxic to cells above freezing temperatures.
More from Cell Culture Reagents
Request a wholesale quote
We have received your message. We will reply by email.