ADCF Serum-Free CHO Feed Supplement Powder

CCM-SF-0030 is a powder-format feed supplement for CHO cell culture processes that require defined, animal-origin-free raw materials. The supplement is serum-free and animal-component-free, supporting controlled media design for R&D scale-up, fed-batch optimization, and biomanufacturing development. It is manufactured without phenol red and without L-glutamine, enabling users to manage indicator background, glutamine strategy, and ammonia-related process variables according to their own platform requirements.

The product is specified for CHO K1, CHO-S, CHO-GS, and CHO DG44 cells under typical mammalian culture conditions of 37 °C and 5–10% CO2. D-glucose is included as a positive additive, making the supplement suitable where feed strategies require an additional carbon source alongside amino acid, nutrient, and process-control planning. The listed format is 500 mL + 50 mL, and the powder form supports controlled preparation, inventory efficiency, and documented lot qualification for repeated process runs.

• Fed-batch supplementation for CHO suspension cultures used in recombinant protein, antibody, antigen, and other biopharmaceutical production workflows.
• Process-development studies comparing feed timing, feed volume, glucose availability, viable cell density, productivity, and metabolite profiles in serum-free media systems.
• Scale-up programs that require animal-origin-free raw materials to reduce undefined inputs and improve lot-to-lot process control.
• CHO K1, CHO-S, CHO-GS, and CHO DG44 culture support in shake flasks, bench bioreactors, and pilot-scale development when qualified by the user’s internal protocol.
• Formulation work where the absence of phenol red supports optical monitoring methods, color-background control, or user-defined pH indicator strategies.
• Media optimization where L-glutamine is added separately, replaced by an alternative glutamine source, or controlled as an independent experimental variable.

Store CCM-SF-0030 at 2–8 °C and protect it from light. The stated shelf life is 24 months when stored under recommended conditions in the original, unopened container. The product ships on blue ice to maintain a controlled cold-chain profile during transit. After receipt, inspect the shipment, record the lot number, and transfer the material promptly to refrigerated storage. During preparation, use aseptic technique and validated water, mixing, filtration, and hold-time procedures appropriate to the receiving laboratory or production environment. Because pH and osmolality targets are not fixed in the supplied specification, they should be confirmed after preparation and any user-defined supplementation steps.

CellCultureMedia supplies CCM-SF-0030 for research and bioprocessing procurement teams that need defined specifications, consistent logistics, and responsive documentation support. The endotoxin specification is ≤20 EU/mL. Sterility status, preparation records, and lot-specific analytical results should be confirmed on the Certificate of Analysis before use in qualified processes. Manufacturing and release documentation is managed under ISO-aligned quality practices; learn more through our quality and documentation resources. For volume planning, standing orders, or controlled resupply programs, procurement teams can request a quote. Free worldwide shipping is available for this SKU, with cold-chain packaging selected according to the stated blue-ice shipping condition.