ADCF ITS-A 100X Supplement with Sodium Pyruvate

CCM-RG-0073 is an animal-origin-free ITS-A 100X cell culture supplement supplied as a sterile-filtered liquid. It is designed for laboratories that need a defined supplement package for serum-reduced, serum-free, or chemically defined media optimization. The formulation provides recombinant human insulin for metabolic support, recombinant human transferrin for iron transport functionality, a specified trace component, and sodium pyruvate as an additional carbon and energy-associated substrate. For teams sourcing related reagents, the product sits within CellCultureMedia's cell culture reagents portfolio.

The listed composition is 1.0 g/L recombinant human insulin, 550 mg/L recombinant human transferrin, 0.67 mg/L sodium nitrite, and 11.0 g/L sodium pyruvate. The liquid specification includes pH 7.0–9.0, osmolality 260–320 mOsmol/kg, endotoxin not more than 1 EU/mL, and sterile filtration. The 100X concentration supports convenient addition during media preparation while limiting dilution of the base formulation. The 10 mL pack size is appropriate for feasibility screening, small-batch R&D media builds, and qualification prior to larger-volume procurement.

• Supplementation of mammalian cell culture media where defined insulin, transferrin, trace component, and pyruvate inputs are required.
• Serum-reduced or serum-free media development for adherent and suspension cell culture workflows.
• Optimization studies comparing defined supplements across candidate basal formulations, feed strategies, or process-development conditions.
• Research-scale preparation of animal-origin-free culture systems for cell line maintenance, expansion, assay preparation, and upstream development.
• Small-batch media preparation in academic, biotech, and biopharma laboratories that require documented sterility, endotoxin, pH, osmolality, and shelf-life information.

Use level should be established by the user for the specific cell type, base medium, and process objective. Because the product is supplied as a 100X concentrate, a typical starting point is a 1:100 dilution into the final medium, followed by mixing under aseptic conditions. For larger programs, procurement teams can request a quote for lot availability, volume planning, and bulk pricing.

Store CCM-RG-0073 at 2–8 °C and protect from light. Do not leave the bottle at room temperature longer than necessary during media preparation. Handle using aseptic technique in a suitable biological safety cabinet or controlled clean workflow to preserve sterility after opening. Mix gently before use; avoid vigorous agitation that can introduce foam or unnecessary air-liquid interface exposure. The stated shelf life is 24 months when stored unopened under recommended conditions. Shipments are dispatched on blue ice to support refrigerated transit, and receiving teams should transfer material to 2–8 °C storage promptly after delivery.

This supplement is manufactured for research and bioprocessing supply chains that require consistent specifications and procurement-ready documentation. Release specifications include sterile-filtered liquid format, animal-origin-free classification, endotoxin ≤1 EU/mL, pH 7.0–9.0, and osmolality 260–320 mOsmol/kg. CellCultureMedia supports ISO-aligned supplier controls, lot traceability, and documentation review through its quality resources.

A Certificate of Analysis is available on request and may include lot-specific results for appearance, pH, osmolality, endotoxin, sterility-related release, and other applicable checks. The listed 10 mL unit price is $319.44, with free worldwide shipping where available. For standing orders, multi-site sourcing, or process-development campaigns, contact CellCultureMedia for quotation support, reserved inventory, and lead-time confirmation.