ADCF HAT Supplement 50X

ADCF HAT Supplement 50X is a concentrated cell culture reagent for use in hybridoma, myeloma fusion partner, and other mammalian cell culture systems requiring hypoxanthine-aminopterin-thymidine selection chemistry. The ADCF designation indicates animal origin-free manufacture, supporting projects where undefined animal-derived inputs must be minimized in upstream development, screening, or bioprocess media design. The product is supplied as a sterile-filtered liquid concentrate for convenient aseptic addition to qualified media.

Key release specifications include liquid form, 50X concentration, sterile filtration, pH 9.0 to 12.0, and a stated shelf life of 24 months when stored as directed. The 100 mL package size supports routine laboratory use as well as scale-up feasibility work. It belongs in the cell culture reagents category and is intended for research, cell line development, and controlled bioprocessing applications. Users should validate final working concentration, media compatibility, and culture performance within their own cell system.

• Selection of fused mammalian cells in hybridoma generation workflows using HAT-compatible media systems.
• Support for cell line development projects requiring defined hypoxanthine, aminopterin, and thymidine supplementation.
• Use in animal origin-free media strategies where reagent traceability and reduced animal-derived inputs are required.
• Optimization of selective culture conditions during small-scale screening, clone evaluation, and process development.
• Supplementation of compatible liquid media after internal verification of pH, osmolality impact, cell growth response, and final formulation performance.

Store CCM-RG-0088 at -30 to -5 °C protected from light. The product ships on dry ice to maintain frozen conditions during transit. On receipt, verify that the container remains frozen or appropriately chilled, record lot details, and transfer promptly to a qualified freezer. Thaw under controlled conditions, mix gently after thawing, and use aseptic technique when dispensing. Avoid repeated freeze-thaw cycles by preparing single-use aliquots when appropriate for the laboratory workflow. Do not use beyond the assigned expiry date or if the container integrity, appearance, or traceability documentation is compromised.

CellCultureMedia supplies this supplement through ISO-aligned quality and supplier qualification processes focused on sterility, lot traceability, documentation control, and cold-chain handling. A Certificate of Analysis is available on request, and additional lot-specific documentation can be coordinated through the quality documentation team when required for vendor qualification or change-control records. Procurement teams can use the request a quote workflow for volume pricing, forecast ordering, standing supply programs, and dry-ice logistics. Free worldwide shipping is available, with packaging selected to support the stated frozen shipping condition.