10X DPBS Without Calcium, Magnesium, or Phenol Red

CCM-RG-0006 is a 10X concentrated DPBS formulation designed for laboratories that require a defined, calcium-free, magnesium-free, and phenol-red-free balanced salt solution. DPBS provides phosphate buffering and inorganic salts to help maintain osmotic balance during routine manipulation of adherent and suspension cultures. The absence of Ca²⁺ and Mg²⁺ supports workflows where divalent cations can influence cell attachment, aggregation, or dissociation performance. The absence of phenol red makes the buffer suitable for optical, fluorescence, and color-sensitive assays where background signal needs to be controlled.

This product is supplied as a sterile-filtered liquid concentrate in a 500 mL format. It is classified as animal origin-free and is intended for research and bioprocessing cell-culture workflows. The source specification lists pH at 7.0–7.4 and osmolality at 270–300 mOsmol/kg; confirm lot-specific values and whether they apply to the concentrate or prepared working dilution on the Certificate of Analysis. For procurement planning, this item sits within our cell culture reagents portfolio and can be requested as a single unit or in larger supply quantities.

• Preparation of 1X DPBS working solution by aseptic dilution with suitable sterile water according to validated internal procedures.
• Washing adherent cell monolayers before dissociation, passaging, transfection setup, or assay preparation when calcium and magnesium are not desired.
• Rinsing suspension cell pellets during media exchange, buffer exchange, or sample preparation steps.
• Use in phenol-red-sensitive imaging, plate-reader, fluorescence, and colorimetric workflows where an indicator dye may add background.
• Buffering of cells, cell-derived samples, or cultureware during short handling steps outside complete growth medium.
• Support for standardized laboratory reagent stocking where a 10X concentrate reduces storage volume and allows preparation of controlled working volumes.

Store unopened containers at 2–30 °C and protect from light. The product is specified for room-temperature shipment, reducing cold-chain complexity for international procurement. Shelf life is 48 months when stored under the stated conditions and used before the labeled expiry date. Inspect the bottle on receipt for seal integrity, clarity, and leakage. After opening, handle aseptically in a controlled environment and avoid repeated contamination risk from pipettes, bottle-top devices, or shared reservoirs. If diluted to 1X, qualify the dilution process, water quality, container compatibility, and in-use hold time under your site’s procedures.

CellCultureMedia supplies CCM-RG-0006 through ISO-aligned manufacturing and quality documentation practices appropriate for research and bioprocess support materials. Each lot is sterile-filtered; sterility status, pH, osmolality, appearance, and other release attributes should be verified on the lot documentation. Endotoxin limits are not stated in the provided specification and should be confirmed before use in endotoxin-sensitive workflows. A Certificate of Analysis is available on request through quality documentation support. For standing orders, multi-site purchasing, or nonstandard quantities, use request a quote; eligible orders include free worldwide shipping.