High-Glucose DMEM with Phenol Red and L-Glutamine

High-glucose DMEM is a widely used classical cell culture medium for adherent mammalian cell systems requiring elevated glucose availability and a bicarbonate-buffered CO2 incubation environment. This 500 mL liquid formulation contains phenol red as a visual pH indicator, 4.5 g/L D-glucose, and 4 mM L-glutamine. It does not contain sodium pyruvate or HEPES, allowing laboratories to control those components separately when protocol-specific supplementation is needed. For comparable media formats and related formulations, see the classical media category.

CCM-CM-0006 is specified for use at 37°C in a 5–10% CO2 incubator and is commonly paired with 10% fetal bovine serum or an equivalent validated supplement, depending on the cell system. Key release parameters include pH 7.0–7.4, osmolality 320–350 mOsmol/kg, endotoxin not more than 1 EU/mL, and sterile filtration. The product is classified as animal-origin-free for the medium formulation itself; users should evaluate the origin and suitability of any added serum or supplements in their own process documentation.

• Routine expansion and maintenance of fibroblasts, smooth muscle cells, and other adherent mammalian cell cultures that are compatible with high-glucose DMEM.
• R&D method development where sodium pyruvate and HEPES are intentionally excluded from the base medium to reduce formulation variables.
• Academic and industrial cell culture projects requiring phenol red for visual monitoring of acidification or alkalinization during incubation.
• Serum-supplemented workflows where the user adds 10% fetal bovine serum, qualified serum alternatives, antibiotics, or additional reagents according to an internal protocol.
• Comparability studies, media screening, and scale-up planning in which pH range, osmolality range, endotoxin limit, and sterility status must be documented for procurement review.

Store CCM-CM-0006 at 2–8°C protected from light. Do not freeze, as freeze-thaw exposure can affect salts, amino acids, and indicator performance in liquid media. The stated shelf life is 12 months when the product remains unopened and stored under recommended conditions. Shipments are packed with blue ice to support temperature-controlled transit; inspect containers on receipt, confirm that the closure is intact, and record lot details before use. Warm only the required volume to working temperature and use aseptic technique to reduce contamination risk after opening.

CellCultureMedia supplies CCM-CM-0006 for research and bioprocessing use under ISO-aligned manufacturing and quality review practices. Each production lot is released against documented specifications for appearance, sterility by filtration process control, pH, osmolality, endotoxin, labeling, and shelf-life assignment. A Certificate of Analysis is available on request through our quality documentation team. For standing orders, multi-pack requirements, or project-based supply planning, submit details through request a quote; qualified orders can include free worldwide shipping.