CHO Serum-Free Dry Powder Medium

CCM-SF-0017 is a dry powder, serum-free medium for CHO suspension culture workflows that require defined input materials, consistent preparation, and scalable procurement. The medium is animal-origin-free and is supplied in a 50 L preparation size for laboratories, pilot groups, and process development teams evaluating CHO expression platforms. It belongs to the CellCultureMedia serum-free media portfolio and is intended for cell culture research, upstream process optimization, and biopharmaceutical production support.

The formulation contains D-glucose and sodium bicarbonate to support carbohydrate availability and carbonate buffering during 37 °C incubation with 5–10% CO2. It is manufactured without serum, phenol red, L-glutamine, or HT supplement, allowing users to control those variables according to an established medium-preparation protocol. Key release specifications include pH 6.9–7.5, osmolality 260–300 mOsmol/kg, and endotoxin not more than 3 EU/mL. These parameters help process engineers compare lots, document medium readiness, and align preparation records with internal batch requirements.

• Serum-free culture of CHO K1, CHO-S, GS-selected CHO, and DG44 CHO host lines in shake flasks, spinner vessels, and controlled bioreactors.
• Upstream process development where animal-origin-free medium is required to reduce undefined raw material inputs and simplify comparability studies.
• Fed-batch and batch screening programs that require a dry powder format for larger-volume preparation, inventory planning, and repeatable media hydration.
• Expression and production-support studies for recombinant proteins, assay reagents, and biologics research materials generated from CHO platforms.
• Medium optimization studies where users may add L-glutamine, HT supplement, feeds, or line-specific supplements under qualified internal procedures, since these are not included in the base formulation.

Store the unopened dry powder at 2–8 °C and protect it from light. The stated shelf life is 24 months when the material is maintained under recommended storage conditions and the container remains sealed. Shipments are arranged with blue ice to maintain controlled transit temperature. During use, prepare the powder with qualified water and validated mixing conditions; because the product is supplied as powder, final-use sterility depends on the user’s reconstitution, filtration, aseptic transfer, and hold-time controls. Confirm final pH and osmolality after preparation and adjustment, especially when adding line-specific supplements.

CellCultureMedia supplies CCM-SF-0017 for B2B procurement teams that require traceable lots, consistent specifications, and responsive documentation. Manufacturing and release controls follow ISO-aligned quality practices, with lot-specific documentation available through our quality documentation process. A Certificate of Analysis can be requested before or after ordering, subject to lot availability. For planned campaigns, standing demand, or multi-site purchasing, submit a request through request a quote to confirm current lot data, lead time, pack configuration, and bulk pricing. Free worldwide shipping is offered where import and transport conditions allow.