CHO 18 Serum-Free ADCF Powder Medium

CCM-SF-0009 is a chemically defined, serum-free powder medium designed for CHO production platforms that require animal-derived component-free inputs. The formulation is supplied as powder for preparation of 50 L of finished medium and includes D-glucose plus HT supplement. It is manufactured without phenol red, which can be useful when optical or colorimetric readouts are part of the workflow, and without L-glutamine, allowing process teams to qualify their preferred glutamine source or substitute during media preparation.

The medium is specified for CHO K1, CHO-S, CHO-GS, and CHO DG44 cell culture under standard conditions of 37 °C and 5–10% CO2. Key release specifications include pH 7.6–8.2, osmolality 260–300 mOsmol/kg, endotoxin ≤3 EU/mL, serum-free classification, and no animal-source classification. The 50 L pack size fits laboratory scale-up, media screening, and pilot preparation where powder logistics and lot documentation are important. For adjacent formats and related formulations, see our serum-free media category.

• Serum-free expansion and maintenance of qualified CHO K1, CHO-S, CHO-GS, and CHO DG44 cell lines.
• Process development for recombinant protein, antibody, vaccine-antigen research, and other biopharmaceutical expression workflows.
• Media evaluation studies where phenol red exclusion and controlled L-glutamine addition are required.
• Scale-up studies from shake flask to controlled bioreactor formats under 37 °C, 5–10% CO2 conditions.
• Procurement programs requiring animal-derived component-free raw materials, defined specifications, and lot-level documentation.

Store the unopened powder at 2–8 °C and protect it from light. The stated shelf life is 24 months when the product is stored as recommended and handled using suitable aseptic procedures. Shipping is performed with blue ice according to the listed transport condition. After reconstitution, use validated site procedures for water quality, mixing order, filtration, pH adjustment, osmolality confirmation, and hold-time assignment. Because the formulation does not contain L-glutamine, add the selected glutamine source only after internal qualification.

CellCultureMedia supplies CCM-SF-0009 for B2B research and bioprocessing procurement with specification-focused support. Manufacturing and release controls are ISO-aligned, and a Certificate of Analysis can be requested for lot traceability, endotoxin confirmation, pH, osmolality, and other applicable release data. Review our documentation approach at quality and compliance, or contact procurement support through request a quote for price confirmation, availability, bulk planning, and free worldwide shipping options.