Chemically Defined 293 Serum-Free Medium Powder

CCM-SF-0060 is a dry-format serum-free medium for high-density 293 suspension culture in chemically defined, animal-derived component-free workflows. The powder format supports centralized media preparation, reduced liquid storage volume, and more efficient inventory planning for laboratories and pilot-scale process groups. It is supplied as a 50 L pack and is suitable for culture conditions of 37 °C with 5–10% CO2. For related options, review the serum-free media category.

The formulation contains D-glucose and is manufactured without phenol red and without L-glutamine. This configuration is useful when users need to add L-glutamine or an alternative glutamine source immediately before use, or when phenol red-free conditions are preferred for optical readouts and downstream analytical compatibility. Key released specifications include powder form, serum-free classification, animal origin-free status, endotoxin ≤3 EU/mL, and 24-month shelf life when stored as directed. pH and osmolality should be verified after reconstitution and final supplementation according to the user’s validated preparation procedure.

• Suspension culture of HEK 293-derived cells used in transient expression, stable expression, and process-development studies.
• Serum-free expansion workflows where lot-to-lot consistency, animal-origin risk reduction, and defined supplementation are procurement requirements.
• Biopharmaceutical process research requiring a phenol red-free medium background and separate control of L-glutamine addition.
• Scale-up evaluations from shake flask to stirred or single-use bioreactor formats, subject to site-specific mixing, feeding, and gas-transfer parameters.
• Raw-material qualification programs where users need clear pack size, endotoxin limit, storage condition, and shelf-life data before placing a production or development order through request a quote.

Store unopened powder at 2–8 °C protected from light. The stated shelf life is 24 months under recommended storage conditions. Shipments are dispatched on blue ice to help maintain controlled transit conditions, and receiving teams should transfer material to refrigerated storage promptly after delivery. Prepare using qualified water and validated mixing procedures, then confirm complete dissolution, pH, osmolality, and filtration or sterility-control steps according to the user’s internal method. Add L-glutamine or other supplements only after compatibility and concentration requirements have been established for the target 293 process.

CellCultureMedia supplies CCM-SF-0060 for research and bioprocessing environments that require documentation-ready specifications and dependable procurement support. Manufacturing and release activities are managed under ISO-aligned quality practices, with lot-specific Certificate of Analysis documentation available on request. Quality documentation can support incoming inspection, supplier qualification, change-control review, and scale-up planning; see quality documentation for additional information. Free worldwide shipping is available, and bulk pricing may be quoted for recurring purchases, multi-pack orders, and program-level supply agreements.