CD 293M Serum-Free Medium

CCM-SF-0059 is a chemically defined-style, serum-free medium intended for 293F-type suspension cell culture workflows where animal component-free inputs, lot traceability, and reproducible handling are procurement requirements. The medium is provided as a ready-to-use liquid and supports research, process development, and biopharmaceutical production support activities involving 293-derived host cells. For teams comparing options in serum-free media, this product offers a simplified additive profile and removes two common formulation variables: phenol red and EDTA.

The formulation contains D-glucose, 2 mM L-glutamine, and 2 mM L-alanyl-L-glutamine. The dual glutamine system provides an immediately available amino acid source plus a more stable dipeptide form for fed-batch or extended shake-flask and bioreactor evaluations. The product is specified at pH 7.0–7.4 and osmolality 270–340 mOsmol/kg, with endotoxin not more than 3 EU/mL. It is sterile-filtered and supplied for culture at 37 °C in 5–10% CO2. The 500 mL pack size supports screening, scale-up bridging, media qualification, and controlled purchasing for multi-site R&D groups.

• Suspension culture of 293F-type and other 293-derived mammalian host cells in serum-free, animal component-free workflows.
• Transient expression studies for recombinant proteins, viral antigens, and process-development materials where phenol red interference is not desired.
• Media comparison studies requiring defined pH, osmolality, endotoxin, and sterility specifications for vendor qualification.
• Shake-flask, spinner, and small bioreactor workflows operated at 37 °C with 5–10% CO2.
• Upstream process development in which EDTA-free formulation chemistry is preferred for downstream compatibility or cell performance evaluation.
• Procurement programs that need a documented 500 mL serum-free medium with consistent storage, shipping, and quality release expectations.

Store CCM-SF-0059 at 2–8 °C protected from light. Do not freeze unless a site-specific validation study has confirmed acceptable performance after thawing. Before use, mix gently to ensure homogeneity and warm only the volume required for the batch or experiment. Use aseptic technique when opening and dispensing, and minimize repeated temperature cycling. The stated shelf life is 12 months when the product is stored unopened under recommended conditions. The product is shipped on blue ice to maintain refrigerated transit conditions; inspect the container on receipt and place it at 2–8 °C promptly.

CellCultureMedia supplies CCM-SF-0059 for research and bioprocessing organizations that require specification-driven purchasing and lot documentation. Manufacturing and release are managed under ISO-aligned quality practices, with sterility, endotoxin, pH, osmolality, appearance, storage, and shelf-life specifications available for review. A Certificate of Analysis can be requested through our quality documentation process, and procurement teams may request current lot availability, volume pricing, or supply planning support through request a quote. Free worldwide shipping is available, with blue-ice shipment used for this refrigerated liquid medium.