ADCF CHO Feed Supplement Without Phenol Red

CCM-SF-0021 is a liquid, animal-derived component-free CHO feed supplement designed for serum-free upstream cell culture. It is intended for use as a supplemental feed rather than as a stand-alone complete growth medium. The formulation is supplied without phenol red, which is useful when optical readouts, colorimetric assays, or process analytics require reduced background from pH indicator dye. It is also supplied without L-glutamine, allowing process teams to control glutamine or alternative dipeptide supplementation separately according to the feeding strategy and cell line requirement.

The supplement contains D-glucose and is specified for CHO K1, CHO-S, CHO-GS, and CHO DG44 workflows, including process development, scale-up studies, and routine bioproduction research. Release specifications include sterile-filtered liquid format, endotoxin not more than 20 EU/mL, pH 6.6 to 6.8, and osmolality of 1200 to 1600 mOsmol/kg. The high osmolality is typical for concentrated feed products and should be considered when defining daily feed volume, bolus timing, and target culture osmolality. For related formulations, see the serum-free media category.

• Fed-batch CHO culture where a defined, serum-free feed is required to support nutrient availability during extended culture duration.
• CHO K1, CHO-S, CHO-GS, and CHO DG44 upstream development at 37 °C with 5 to 10% CO2 incubation conditions.
• Animal origin-free process models where raw material traceability and reduced undefined components are important procurement criteria.
• Phenol red-free workflows involving absorbance, fluorescence, imaging, or metabolite monitoring where dye interference should be minimized.
• Glutamine-controlled feeding programs in which L-glutamine, stable glutamine substitutes, or nitrogen sources are managed as separate process variables.
• Biopharmaceutical and vaccine-related research manufacturing workflows requiring consistent feed concentrate specifications, sterile filtration, and documented release criteria.

Store CCM-SF-0021 at 2 to 8 °C protected from light. Do not leave the container at room temperature longer than necessary during preparation, and use aseptic technique when transferring or aliquoting the supplement. The product is shipped on blue ice to maintain refrigerated transit conditions; inspect the bottle on arrival for closure integrity, clarity, labeling, and lot identification. The stated shelf life is 12 months when stored unopened under recommended conditions. Because the product is a concentrated feed with osmolality of 1200 to 1600 mOsmol/kg, addition rates should be validated in the user’s culture system to avoid unintended osmotic shifts.

CellCultureMedia supplies CCM-SF-0021 for research and bioprocessing customers who require specification-driven purchasing, lot documentation, and responsive order support. Manufacturing and release controls are aligned with ISO-style quality practices, including sterile filtration and analytical checks for pH, osmolality, endotoxin, appearance, and shelf-life assignment. A Certificate of Analysis is available on request, and additional documentation can be coordinated through the quality documentation team. For planned campaigns, multi-bottle purchases, standing orders, and site-to-site supply planning, submit requirements through request a quote. Free worldwide shipping is available where logistics and destination requirements permit.