Xeno-Free MSC Expansion Medium, Phenol Red-Free

CCM-SC-0014 is designed for expansion of human mesenchymal stem cells in serum-free, xeno-free culture systems. The medium is supplied as a ready-to-use liquid and is manufactured without phenol red, making it suitable for workflows where optical readouts, assay background, or visual indicator components need to be minimized. The product is classified as a serum-free medium and contains an FBS substitute, enabling users to maintain a defined culture approach while avoiding direct serum supplementation.

Key release specifications include pH 7.0-7.4, osmolality 270-340 mOsmol/kg, endotoxin not more than 3 EU/mL, and sterile-filtered processing. The recommended culture environment is 37°C with 5-10% CO2. This medium is aligned with procurement needs for cell and gene application development, academic stem cell laboratories, and bioprocess groups standardizing hMSC expansion inputs. Related products for stem cell workflows are listed in the stem cell media category.

• Expansion culture of human mesenchymal stem cells under serum-free and xeno-free conditions.
• Phenol-red-free culture systems where reduced color interference is required for imaging, absorbance-based assays, or medium monitoring protocols.
• Process development studies comparing defined media, FBS substitute systems, and serum-free culture inputs.
• Research-scale cell and gene application development workflows requiring documented pH, osmolality, endotoxin, and sterility specifications.
• Academic, biotechnology, and biopharma R&D programs evaluating hMSC growth, morphology, and culture consistency across passages.

Store CCM-SC-0014 at -30°C to -5°C protected from light. The product is shipped on dry ice and should be transferred to appropriate frozen storage immediately on receipt. Avoid repeated freeze-thaw cycles; thaw only the volume required for validated internal procedures where possible. The stated shelf life is 12 months when stored under recommended conditions in the original container. Use aseptic technique when handling, and inspect the container and liquid appearance before use according to your laboratory acceptance criteria.

CellCultureMedia supplies CCM-SC-0014 for research and bioprocessing use with specification-focused documentation for purchasing, QA intake, and technical evaluation. Manufacturing and release practices are ISO-aligned, and a Certificate of Analysis can be requested for lot-specific review, including sterility, endotoxin, pH, and osmolality where applicable. For documentation packages, standing supply, or multi-site purchasing, contact our team through the request quote page. Additional information about quality systems and document availability is provided on the quality page. Free worldwide shipping is available for qualifying dry-ice shipments.