ST Cell Serum-Free ADCF Powder Medium

CCM-SF-0050 is supplied as a dry powder medium for preparation of 50 L of serum-free ST cell culture medium. It is designed for suspension or adherent ST cell work where users require a consistent formulation, reduced serum variability, and simplified inventory management versus ready-to-use liquid formats. The product is specified for incubation at 37 °C with 5-10% CO2, supporting standard laboratory and process-development culture environments.

Key formulation components include phenol red for visual pH indication, D-glucose as a carbohydrate source, 4 mM L-glutamine, and 2 mM L-alanyl-glutamine for added glutamine stability during preparation and use. Release specifications include pH 3.5-4.5 for the supplied product, osmolality 240-320 mOsmol/kg after preparation as specified, and endotoxin not more than 3 EU/mL. The supplied classification lists animal origin; teams with animal-component-free requirements should review the lot documentation and project specifications before qualification. Related products are available in our serum-free media category.

• Serum-free culture of ST cells for R&D, scale-up studies, and controlled process-development experiments.
• Biopharmaceutical and vaccine-production research workflows requiring documented medium chemistry, repeatable powder reconstitution, and defined lot release testing.
• Serum-reduction programs where laboratories are moving away from undefined serum inputs while maintaining compatibility with ST cell culture parameters.
• Process engineering evaluations comparing powder medium logistics, storage footprint, hydration procedures, and prepared-medium performance across multiple runs.
• Procurement standardization for academic, biotech, and biopharma groups that need a 50 L format, formal quoting, and batch documentation. For project pricing, use the request quote page and reference CCM-SF-0050.

Store the unopened powder at 2-8 °C protected from light. The stated shelf life is 24 months when stored under recommended conditions and handled according to site procedures. Shipments are packed on blue ice to protect temperature exposure in transit. After receipt, verify package integrity, record lot information, and transfer material promptly to refrigerated storage. During reconstitution, use qualified water and validated mixing, filtration, and pH/osmolality confirmation steps defined by your internal SOPs. Prepared medium handling conditions should be established by the end user based on filtration method, container closure, hold time, and application-specific acceptance criteria.

CellCultureMedia supplies CCM-SF-0050 for research and bioprocessing procurement programs that require clear specifications, lot traceability, and documentation support. Manufacturing and release controls are managed within ISO-aligned quality practices, with Certificate of Analysis availability on request for qualified orders. Typical documentation may include lot number, formulation class, endotoxin limit, pH range, osmolality range, shelf life, storage conditions, and shipping conditions. Review our quality information for documentation expectations. Free worldwide shipping is available, and bulk or scheduled supply can be quoted for multi-site or campaign-based purchasing.